Supporting information for Zhang et al. (2003) Proc. Natl. Acad. Sci. USA, 10.1073/pnas.242719399

Supporting Figure 6

Fig. 6.

 Scheme of a portion of the main control region of human mtDNA, showing the positions of the segments into which it was subdivided for PCR, denaturing gradient gel electrophoresis (DGGE), cloning, and sequencing analysis. OH1, OH2: primary and secondary origin of H-strand synthesis; H1, H2, and L: initiation sites for, respectively, transcription of rRNA-encoding H-strand DNA; transcription of whole H-strand; and transcription of L-strand and synthesis of primer for H-strand synthesis (1); CSB1, CSB2, and CSB3: conserved sequence blocks 1, 2, and 3 (2). The map positions of the DLP4 and DLP6 segments chosen for analysis in this work, of the overlapping DLP3, DLP7, and TRNA1 segments (3) and of the large Init-Tra-Rep fragment, encompassing all the other fragments shown (3) used for PCR and cloning are also shown. Phe: tRNAphe gene.

 

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