Supporting information for Gelling et al. (2003) Proc. Natl. Acad. Sci. USA, 10.1073/pnas.0237106100
Fig. 6.
Disruption of the mouse glucagon receptor locus. (A) Gene-targeting scheme for disruption of the mouse glucagon receptor locus (Gcgr). Structure and partial restriction map of the wild-type locus, targeting vector, and mutated locus. Black and clear boxes indicate coding and noncoding exons, respectively. HI, BamHI; BII, BglII; E, EcoRI; X, XbaI; S, SfiI. Position of the outside probe used to confirm targeting, neoR, and thymidine kinase cassettes are also indicated. (B) Southern blot analysis of HindIII digested genomic DNA. Genomic DNA from WW6 embryonic stem cells (ES) cells, one of three correctly targeted clones (Left), and mouse tail DNA (Right). Position and size of the endogenous and targeted alleles are 15.5 and 8.5 kb, respectively. Genotype is indicated above each lane. An additional band seen in the targeted WW6 ES cell line due to nonspecific hybridization of the probe to a high copy number globin transgene (indicated at left) (11) was selectively bred out of the Gcgr+/– mouse lines.