Blood, Vol. 116, Issue 17, 3297-3310, October 28, 2010
Spatiotemporal organization, regulation, and functions of tractions during neutrophil chemotaxis
Blood Shin et al. 116: 3297
Supplemental materials for: Shin et al
Files in this Data Supplement:
- Document 1. Supplemental methods and figures (PDF, 1.53 MB)
- Video 1 (Traction map) the responses of control dHL-60 cells to a point source of fMLP (MOV, 4.7 MB) -
Some of the time points are shown in Fig. 1A in the text. The total time is 290 sec. - Video 2. (Fluorescence channel) the responses of dHL-60 cells expressing mCherry-tagged myosin IIA to a point source of fMLP (MOV, 570 KB) -
Some of the time points are shown in Fig. 2B in the text. - Video 3. (DIC channel) the responses of dHL-60 cells expressing mCherry-tagged myosin IIA to a point source of fMLP (MOV, 761 KB) -
Some of the time points are shown in Fig. 2B in the text. - Video 4. (Green fluorescence channel) 3-dimensional views of a polarized dHL-60 cell stained with Alexa fluor 488 phalloidin to localize F-actin (green) (MOV, 2.95 MB) -
The schematic views are shown in Fig. S2B. - Video 5. (Red fluorescence channel) 3-dimensional views of a polarized dHL-60 cell stained with Alexa fluor 488 phalloidin to localize a specific antibody against myosin IIA (red) (MOV, 2.63 MB) -
The schematic views are shown in Fig. S2B. - Video 6. (DIC channel) the responses of control dHL-60 cells to a point source of fMLP (MOV, 2.33 MB) -
Some of the time points are shown in Fig. 2F in the text.
Additional supplemental videos can be found here.