Bacillus subtilis CwlP of the SP-β Prophage Has Two Novel Peptidoglycan Hydrolase Domains, Muramidase and Cross-linkage Digesting dd-Endopeptidase

Supplemental Data

Files in this Data Supplement:

• Supplemental Fig legends (.pdf, 36 KB) -

  Supplemental Figure S1. Cell wall hydrolytic activity of CwlP-SLT, CwlP-M23, CwlP-SLTM23 and full-length CwlP. Supplemental Figure S2. ESI-MS analysis of the peak 2 material shown in Fig. 2A. Supplemental Figure S3. ESI-MS and -MS/MS analyses of the peak 3 material shown in Fig. 2A. Supplemental Figure S4. Determination of cleavage sites for CwlP-M23 and LytF by RP-HPLC. Supplemental Figure S5. Hydrolysis of Escherichia coli cells with CwlP-SLT and CwlP-M23 proteins.

• Supplemental Figs. S1-S5 (.pdf, 168 KB) -

  Supplemental Figure S1. Cell wall hydrolytic activity of CwlP-SLT, CwlP-M23, CwlP-SLTM23 and full-length CwlP. Supplemental Figure S2. ESI-MS analysis of the peak 2 material shown in Fig. 2A. Supplemental Figure S3. ESI-MS and -MS/MS analyses of the peak 3 material shown in Fig. 2A. Supplemental Figure S4. Determination of cleavage sites for CwlP-M23 and LytF by RP-HPLC. Supplemental Figure S5. Hydrolysis of Escherichia coli cells with CwlP-SLT and CwlP-M23 proteins.

• Supplemental Table S1-S3 (.pdf, 110 KB) -

  Supplemental Table S1. Plasmids used in this study Supplemental Table S2. Primers used in this study Supplemental Table S3. Hydrolytic activities of CwlP-SLT, CwlP-M23 and their point-mutated proteins using zymography