TRM1, a YY1-like suppressor of rbcS-m3 expression in maize mesophyll cells -- Data Supplement - Supplemental Figures -- Proceedings of the National Academy of Sciences

Supplementary material for Xu et al. (2001) Proc. Natl. Acad. Sci. USA 98 (5), 2295–2300. (10.1073/pnas.041610098)

Fig. 11.
Overexpression of GST-TRM1 fusion protein. The expression and subsequent digestion of the fusion protein with thrombin was monitored by SDS/PAGE. (A) Induction of expression of TRM1 in E. coli. Lane 1, High-range prestained protein molecular weight standards (GIBCO/BRL); lane 2, uninduced pGEX-KG-TRM1 plasmid containing cells; lane 3, pGEX-KG-TRM1 containing cells after 2.5 h of induction; and lane 4, pGEX-KG vector plasmid containing cells after 2.5 h of induction . (B) Purified E. coli-expressed TRM1. Lane 1, Low-range prestained protein molecular weight standards (GIBCO/BRL); lane 2, TRM1 after purification by glutathione-Sepharose 4B affinity chromatography and cleavage with thrombin.