Quantitative PCR-Based Competitive Index for High-Throughput Screening of Salmonella Virulence Factors
Infect. Immun. Yoon et al. 79: 360
Supplemental material
Files in this Data Supplement:
- Supplemental file 1 - Table S1. List of 17 regulators inferred to have a virulence function and their deletion phenotypes concerned with virulence in mice.
Table S2. Barcode (24-nt) sequences replacing 17 regulator genes and a reference gene.
Fig. S1. Virulence test of ΔSTM0314.
Fig. S2. PCR amplification efficiency values of strains tested in this study.
Fig. S3. Specificity of barcode primers in a mixed population.
Fig. S4. Comparison of CIqPCR analyses of 18 strains in spleen and liver in Nramp1+/+ and Nramp1−/− mice.
Fig. S5. Time course CIqPCR analysis of 18 strains in Nramp1+/+ and Nramp1−/− BMDM.
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