Quantitative PCR-Based Competitive Index for High-Throughput Screening of Salmonella Virulence Factors
Infect. Immun. Yoon et al. 79: 360

Supplemental material

Files in this Data Supplement:

  • Supplemental file 1 - Table S1. List of 17 regulators inferred to have a virulence function and their deletion phenotypes concerned with virulence in mice.
    Table S2. Barcode (24-nt) sequences replacing 17 regulator genes and a reference gene.
    Fig. S1. Virulence test of ΔSTM0314.
    Fig. S2. PCR amplification efficiency values of strains tested in this study.
    Fig. S3. Specificity of barcode primers in a mixed population.
    Fig. S4. Comparison of CIqPCR analyses of 18 strains in spleen and liver in Nramp1+/+ and Nramp1−/− mice.
    Fig. S5. Time course CIqPCR analysis of 18 strains in Nramp1+/+ and Nramp1−/− BMDM.
    MS Word document, 1.7 MB.