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- Figure S1 - Characterization of the anti-AT1R and anti-AT2R antibodies. To confirm antibody specificity, two non-pace samples were subjected to immunoblotting with either anti-AT1R (panel A) or anti-AT2R (panel B) antibodies (red) with and without blocking peptides. To confirm protein expression, all blots were also labeled with anti-GAPDH antibody (green). Immunoreactive bands seen in red at approximately 50 and 45 kDa, representing AT1R and AT2R respectively, were detected in positive controls but not detected on blots that were incubated with blocking peptide. These data confirm specificity of the commercially available AT1R and AT2R antibodies.