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Fig. S1. Three-dimensional reconstruction of eyeless-, distal-less- and apterous-positive cell expression during larval development. (A-C) Three-dimensional reconstruction from confocal sections (rendered using Volocity software) of a Drosophila late third instar larval immunostained brain showing the expression patterns of ey-Gal4 driving UAS-nuGFP (red), Distal-less (green), ap-lacZ (gray) and DE-Cad (blue) to reveal the neuroepithelium. Arrows indicate the direction of delamination of ap- (white), dll- (blue) and ey-positive cells (red) from the OPC to generate the medulla cortex.
Fig. S2. Assembly of the medulla cortex circuit. (A-F) Expression patterns of ey-Gal4 driving UAS-nuGFP (green), Distal-less (red) and ap-lacZ (blue) in cell populations in anterior (A-C) and middle sections (D-F) at L3 (A), P0 (B), P20 (C), P40 (D), P68 (E) and adult stages (F). Dashed lines in D-F delineate the prospective medulla rim cells.
Fig. S3. Expression pattern of apterous and distal-less in OPC-derived cells. (A-D) Expression pattern of ap-Gal4 (A,B) and dll-Gal4 driving UAS-CD8-GFP (C,D) in OPC-derived postmitotic cells. OPC-derived neuroblasts were visualized with Miranda (red), neuroepithelial cells with DE-Cadherin (blue) and nuclei with DAPI (gray).