Among Developmental Regulators, StuA but Not BrlA Is Essential for Penicillin V Production in Penicillium chrysogenum
Appl. Environ. Microbiol. Sigl et al. 77: 972
Supplemental material
Files in this Data Supplement:
- Supplemental file 1 - Detailed description of the construction of all used transformation fragments.
MS Word document, 41K. - Supplemental file 2 - Protein sequence alignment of P. chrysogenum BrlA with the corresponding amino acid sequences of A. nidulans AnBrlA and A. fumigatus AfBrlA (Fig. S1); protein sequence alignment of P. chrysogenum StuA with the corresponding amino acid sequences of A. flavus AflStuA, Neosartorya fischeri NfStuA, A. nidulans AnStuA, and A. fumigatus AfStuA (Fig. S2); generation of the P. chrysogenum gene deletion strains ΔbrlA and ΔstuA, the respective complemented strains with genes brlA-kom and stuA-kom, the stuA-overexpressing strain stuA-OE, and the promoter-reporter-gene GUS and GUS-m mutants (Fig. S3); dry weight of strains with mutations ΔbrlA and ΔstuA grown in production medium for 96 h compared to the niaD18 and Δku70 mutants (Fig. S4); regulatory network controlling conidiogenesis in P. chrysogenum (Fig. S5); expression of the reporter gene uidA in the GUS and GUS-m strains measured by qRT-PCR (Fig. S6).
Zipped PDF file, 901K. - Supplemental file 3 - Fungal strains used in this study (Table S1).
MS Word document, 33K. - Supplemental file 4 - Plasmid constructs employed in this study (Table S2).
MS Word document, 38K. - Supplemental file 5 - Oligonucleotides used in this study (Table S3).
MS Word document, 75K. - Supplemental file 6 - Genes deregulated ≥3-fold at ≥3 consecutive time points in both ΔbrlA and ΔstuA compared to Δku70 (Table S4).
MS Word document, 250K. - Supplemental file 7 - Ninety-three genes similarly regulated to abaA in both mutant strains compared to Δku70 (Table S5).
MS Word document, 111K. - Supplemental file 8 - Thirty-eight genes similarly regulated to wetA in both mutant strains compared to Δku70 (Table S6).
MS Word document, 59K.