Blood, Vol. 116, Issue 26, 6092-6100, December 23, 2010

RGS/G_i2 interactions modulate platelet accumulation and thrombus formation at sites of vascular injury
Blood Signarvic et al. 116: 6092

Supplemental materials for: Signarvic et al

Files in this Data Supplement:

• Figure S1. Increased thrombus area in G_i2α(G184S/G184S) radiation chimera mice following laser injury in cremaster muscle arterioles (JPG, 56.5 KB) -
  Mean thrombus area vs. elapsed time after laser injury. The data set included 22 injuries observed in 4 mice transplanted with liver-derived cells from G_i2α(G184S/G184S) embryos and 22 injuries in 4 mice transplanted from wild-type embryos.
  
  
  
  
  
  
• Figure S2. Normal α-granule secretion in platelets from G_i2α(+/G184S) mice (JPG, 96.1 KB) -
  G_i2α(+/G184S) and G_i2α(+∕+) platelets were incubated with the PAR4 agonist peptide, AYPGKF at the concentrations indicated. The appearance of the a-granule membrane protein, P-selectin, on the platelet surface was detected by flow cytometry. The results shown are representative of 3 experiments.
  
  
  
  
  
  
• Video 1. Thrombus formation in G_i2α(+∕G184S) mice following laser injury in cremaster muscle arterioles (MOV, 4.76 MB) -
  Time resolved thrombus formation in G_i2α(+∕G184S) following laser injury. Platelets are labeled with fluorescently-conjugated anti-CD41 Fab fragments (green) superimposed on the brightfield image of surrounding vasculature. Movies shown are representative of the median integrated fluorescence intensity plot derived from all measured injuries (Fig. 1B). The data set included 38 injuries observed in 5 G_i2α(+∕G184S) mice.
  
  
• Video 2. Thrombus formation in G_i2α(+∕+) mice following laser injury in cremaster muscle arterioles (MOV, 4.82 MB) -
  Time resolved thrombus formation in G_i2α(+∕+) following laser injury. Platelets are labeled with fluorescently-conjugated anti-CD41 Fab fragments (green) superimposed on the brightfield image of surrounding vasculature. Movies shown are representative of the median integrated fluorescence intensity plot derived from all measured injuries (Fig. 1B). The data set included 33 injuries in 4 matched controls G_i2α(+∕+).