Kre6 Protein Essential for Yeast Cell Wall β-1,6-Glucan Synthesis Accumulates at Sites of Polarized Growth

Supplemental Data

Files in this Data Supplement:

• Supplementary Figures 1-4 (.pdf, 2.8 MB) - Supplementary figures.
• Figure 6 (.jpg, 313 KB) - FIGURE 6. Immunofluorescent images of Kre6 in comparison with the SV/PM-marker Snc1.
• Figure 2 (.jpg, 73 KB) - FIGURE 2. Specificity of rabbit anti-Kre6 antiserum.
• Figure 4 (.jpg, 229 KB) - FIGURE 4. Sucrose density gradient fractionation of Kre6-containing compartments.
• Figure 1 (.jpg, 284 KB) - FIGURE 1. Localization of wild-type Kre6 and the tagged Kre6-3HA proteins expressed from the chromosomal genes.
• Figure 5 (.jpg, 286 KB) - FIGURE 5. Immunofluorescent images of Kre6 in Comparison with the ER-marker Lip1.
• Figure 8 (.jpg, 185 KB) - FIGURE 8. Deletions including possible ER-exit motifs in the cytoplasmic domain and the biological activity of Kre6.
• Figure 3 (.jpg, 77 KB) - FIGURE 3. Topology analysis of Kre6-3HA protein by protease protection assay.
• Figure 7 (.jpg, 9.0 MB) - FIGURE 7. Localization of Kre6-3HA decided by immunoelectron microscopic observation.