Supplemental Data
Files in this Data Supplement:
- Supplemental FIgure 1 (.pdf, 3.4 MB) - Microbial rhodopsins from various species of halobacteria. Alignment of amino acid sequences of BRs (9 genes at the top) and SRIIs (3 genes at the bottom) reported so far with HwBR and MR was performed by ClustalW. Names of microbial rhodopsins and sources from the top are: bop_por, port BR (D11057); co3_Hva, Ha. v. cruxrhodopsin-3 (D31882); bop_sha, shark BR (D11058); bop_SGl, Hb. sp. strain SGI BR (X70291); ao3-Hso, Halorubrum sodomense archaerhodopsin-3 (D50848); ao2-au2, Hb. sp. strain aus-2 archaerhodopsin-2 (S56354); bop-mex, mex bR (D11065); bop-S9, S9 BR (J01727); bopII_Hw, Haloquadrutam walsbyi BR (YP_656801); bopI_Hw, Haloquadratum walsbyi opsin (YP_656804); sopII_Nph, N. pharaonis sop (J05199); sopII_Hva, Ha. v. pR (Z35308); sopII_F15, Flxl5 pR (U62676). bop_S9 BR from Halobacterium salinarum and sopII_Nph SRII from Natronomonas pharaonis correspond to BR and SRII, respectively, used in this study.
- Supplemental Figure S2 (.pdf, 616 KB) - Putative secondary structure of MR (bopI). The white letters on the black background indicate highly conserved residues among microbial rhodopsins, Asp84 and Lys217.