Rotational Position of a 5-Methylcytosine-containing Cyclobutane Pyrimidine Dimer in a Nucleosome Greatly Affects Its Deamination Rate

Supplemental Data

Files in this Data Supplement:

• Supplementary Figures 1-6 (.pdf, 393 KB) - Contains Supplementary Figures 1 - 6 Fig. S1. Ligation strategy for assembly of the 150-mer top strand substrates. Fig. S2. Sequences used to assemble the 150-mer top and bottom strands as shown in Fig. S1. Fig. S3. Characterization and purification of the 150-mer DNA duplexes by PAGE. Fig. S4. Reconstitution of the nucleosome core particles with the 150-mer duplexes. Fig. S5. Linear regression analysis of the deamination rate data for CPD-IN. Fig. S6. Linear regression analysis of the deamination rate data for CPD-OUT.