Supplementary material for Silverman et al. (2001) Proc. Natl. Acad. Sci. USA 98 (6), 3092–3097. (10.1073/pnas.041613598)

 Table 4. Shuffled library selection data

Mutant

P

 (mutant)*

No. of sequences†

Buried polar interactions

0.5

27

Secondary structure punctuation

0.4

20

b

-central residues

0.7

9

Diamond residues

0.6

14

Barrel surface residues (nonvaline)

0.3

19

Barrel interior (nonvaline)

0.5

19

Miscellaneous

0.6

16

*Highest fraction of mutant sequence found to produce functional shuffled sequences.

†

Number of independent clones sequenced from each library.