Glycine Hinges with Opposing Actions at the Acetylcholine Receptor-Channel Transmitter Binding Site

Data Supplement

Files in this Data Supplement:

• Data Supplement - Table S1: Diliganded rate and equilibrium constants.

  Table S2: Unliganded rate and equilibrium constants.

  Table S3: Estimation of net agonist binding and gating energies.

  Table S4: Estimation of R and R* affinities for alphaG147 and alphaG153 mutants.

  Table S5: Unliganded gating of alphaG153S mutants.

  Supplemental References.

• Data Supplement - Supplemental Figure 1: Example single channel current traces for loop B residues examined in this study.
• Data Supplement - Supplemental Figure 2: Example traces and shut/open histograms of loop B mutants alphaG147A, alphaG147S, alphaW149C and alphaW149A.
• Data Supplement - Supplemental Figure 3: Correlation between fold-changes in E2 and E0 for alphaG153 mutants.
• Data Supplement - Supplemental Figure 4: Global R/E analyses of all loop B residues.