Arp2/3 complex and actin dynamics are required for actin-based mitochondrial motility in yeast -- Data Supplement - Supplemental Data -- Proceedings of the National Academy of Sciences

Supplementary material for Boldogh et al. (2001) Proc. Natl. Acad. Sci. USA 98 (6), 3162–3167. (10.1073/pnas.051494698)

Fig. 6.
Localization of Arc15p in yeast. To localize Arc15p, we tagged its C terminus with the Myc epitope. This tag does not appear to compromise Arc15p function: cells expressing Arc15p-myc show normal growth rates, actin organization, and mitochondrial motility. Visualization of Arc15p and mitochondria in yeast fixed with formaldehyde alone was carried out by immunofluorescence using anti-myc antibodies to detect myc-tagged Arc15p (B), and antibodies raised against total mitochondrial outer membrane proteins (A). Immunostaining reveals punctate distribution of Arc15-myc in the growing bud and in the mother cell. Arc15p-containing structures are more numerous and uniformly distributed than Arp2p structures. This finding suggests that Arc15p is assembled into punctate, presumably macromolecular, complexes and that Arp2p is present in some, but not all, Arc15p-containing structures. Similarly, some, but not all, Arc15p-containing structures appear to colocalize with mitochondria. (Bar = 1 µm.)