Fig. S1. Rec24−GFP and Rec7−GFP are induced normally in rec10Δ, rec12Δ, Δrec7Δ and rec24Δ mutants. Diploid pat1-114 rec24−GFP or pat1-114 rec7−GFP cells with the indicated deletions (strains CMC197, CMC287, CM291, CMC304 and CMC271, CMC272, CMC273) were induced for meiosis and collected for protein extraction at the indicated times. Between 1.5 and 2 hours after meiotic induction the bulk of the cells were in S phase in all the cultures (unpublished data). Western blots were incubated with anti−GFP antibodies and anti-actin antibodies for a loading control.

Fig. S2. Kinetics of Rec24−GFP and Rec7−GFP chromosome loading. (A) Distribution of the number of Rec24−GFP foci and Rec7−GFP foci at 2.5 and 3 hours after meiotic induction. Data, corresponding to the experiments shown in Fig. 4B and Fig. 6, are the percentage of Rec10-positive nuclei with the indicated number of GFP foci. The numbers of Rec10-positive nuclei analyzed were, for Rec24−GFP and Rec7−GFP, respectively, 65 and 45 at 2.5 hours and 68 and 45 at 3 hours. Similar distribution of Rec7−GFP foci at maximal chromosomal loading was observed in an independent meiosis. (B) Rec24−GFP and Rec7−GFP focus formation in parallel meioses. Diploid pat1-114 rec24−GFP and pat1-114 rec7−GFP cells (strains CMC197 and CMC271) were induced for meiosis and collected for nuclear spread preparation at the indicated time points. The percentage of Rec10-positive nuclei containing GFP foci is represented. Data are cumulative numbers of two independent stains of the same meiotic induction, except for 3.5 hours in which a single stain was done. The number of nuclei analyzed (Rec10-positive/total nuclei) at each time point and genotype are shown on top of the corresponding bar.

Table S1. Rec24-GFP, Rec24-HA and Rec7-GFP fusion proteins are functional.

Table S2. Strains used.