Reconstitution of the FK228 Biosynthetic Pathway Reveals Cross Talk between Modular Polyketide Synthases and Fatty Acid Synthase -- Wesener et al. 77 (4): 1501 Data Supplement - Supplemental material -- Applied and Environmental Microbiology

Reconstitution of the FK228 Biosynthetic Pathway Reveals Cross Talk between Modular Polyketide Synthases and Fatty Acid Synthase
Appl. Environ. Microbiol. Wesener et al. 77: 1501

Supplemental material

Files in this Data Supplement:

Supplemental file 1 - Primers used for gene deletion, genotype detection, and detection of gene expression (Table S1), quantification of FK228 production by recombinant E. coli strains relative to the wild-type strain of C. violaceum no. 968 cultivated under aerobic conditions (Table S2), 16S rRNA gene sequence alignment for C. violaceum no. 968 and ATCC 12472 by CLUSTAL W (Fig. S1), examination and quantification of FK228 production by LC-MS (Fig. S2), and alignment of FabD sequences of C. violaceum no. 968 and E. coli BL21(DE3) by CLUSTAL W (Fig. S3).
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