Natural Competence in the Hyperthermophilic Archaeon Pyrococcus furiosus Facilitates Genetic Manipulation: Construction of Markerless Deletions of Genes Encoding the Two Cytoplasmic Hydrogenases -- Lipscomb et al. 77 (7): 2232 Data Supplement - Supplemental material -- Applied and Environmental Microbiology

Natural Competence in the Hyperthermophilic Archaeon Pyrococcus furiosus Facilitates Genetic Manipulation: Construction of Markerless Deletions of Genes Encoding the Two Cytoplasmic Hydrogenases
Appl. Environ. Microbiol. Lipscomb et al. 77: 2232

Supplemental material

Files in this Data Supplement:

Supplemental file 1 - Plasmid constructs used in this study (Fig. S1); PCR screen showing plasmid integration at three locations in simvastatin-resistant isolates (Fig. S2); qPCR amplification plots with primers specific to pyrF (Fig. S3); PCR screens of transformants from transformation with a PCR product or genomic DNA (Fig. S4); PCR screens for SHI and SHII operon deletions (Fig. S5); growth curves of ΔSHI, ΔSHII, and ΔSHI ΔSHII mutants (Fig. S6); primers used in this study (Table S1).
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