Table 3. RT-PCR primer sequences
|
Genes |
|
Sequence of forward and reverse primers (5' to 3') |
Accession no. |
|
HSL |
Sense |
GTGTTCGGGAACAGGCACTGGGGCTAG |
U40001 |
|
|
Antisense |
GAAGCCCAGGCAGCGACCGTAAAAGCAG |
|
|
FATP |
Sense |
GCAGGAACAGCCTCTGTGGCCTCATTGGGG |
U89529 |
|
|
Antisense |
GTAGGTGTCTAGCTGTGCGAAGGTCCAG |
|
|
CPT1 |
Sense |
GAGCCACGAAGCCCTCAAACAGAT |
L07736 |
|
|
Antisense |
GCTGTACAACATGGGCTTCCGACCTG |
|
|
LCAD |
Sense |
GGCAATTTCTGCCTGTGAGTTCATGTTTG |
J05029 |
|
|
Antisense |
GTCACTGACGATCTGTCTTGCGATCAG |
|
|
HADHA |
Sense |
GGAGCGCCGTCCTTATTTCGTCAAAG |
D16478 |
|
|
Antisense |
GGTAAGATTCCCAGCAACACTTCAGGG |
|
|
DECR |
Sense |
GGTCCTCAGAAGTTTTTCAGCTTTGGAACG |
D00569 |
|
|
Antisense |
GGGAGAAATGAAGTTCCCTGCCGCATTG |
|
|
Thiolase |
Sense |
GCAGGGAACGCCTCGGGCATGTCTGACGG |
X05341 |
|
|
Antisense |
GATCCCACTGCGTATTTTCCACCTCGACG |
|
|
HMGCS2 |
Sense |
GGCGGGTCCTGCAAGTGAAGAGAGTCATG |
M33648 |
|
|
Antisense |
GATGTCAGTGTTGCCTGAATCCTGGAAG |
|
|
UCP2 |
Sense |
TCATGGTTGGTTTCAAGGCCACCGATG |
AB005143 |
|
|
Antisense |
GCTCAAAAGGGTGCCTCCCGGGATTCA |
|
|
UCP3 |
Sense |
ACCATGGTTGGACTTCAGCCATCAG |
AF035943 |
|
|
Antisense |
AAAGGAACGGACCACTCAGCGTCG |
|
|
PDK4 |
Sense |
GCTGTCCATGAAGCAGCTGCTGGACTTCGG |
AF034577 |
|
|
Antisense |
GGTCCAAAAAATACTGAAGATTTTGATTGG |
|
|
Rab11B |
Sense |
GGACAATGGGGACCCGGGACGACGAG |
D01046 |
|
|
Antisense |
GCAGTCACAGGTTCTGGGAGCACTG |
|
|
Myogenin |
Sense |
GTAAGAGGAAGTCTGTGTCTGTGG |
M24393 |
|
|
Antisense |
GTGATGCTGTCCACGATGGACGTAAGG |
|
|
PGC-1a |
Sense |
GTATGGAGTGACATAGAGTGTGCTGCCCTG |
AB025784 |
|
|
Antisense |
GGTTTGTTCTGATCCTGTGGGTGTGGTTTG |
|
|
GAPDH |
Sense |
GTGAAGGTCGGTGTCAACGGATTTGG |
M17701 |
|
|
Antisense |
GACCCTTTTGGCACCACCCTTCAGG |
|