Table 4. Real-time PCR primer sequences
|
Genes |
|
Sequence of forward and reverse primers (5' to 3') |
GenBank |
|
FATP |
Sense |
CAGGCACCTTCAAGATCCAGAA |
AF023256 |
|
|
Antisense |
TACATAGCGTCCCTGCTTCAGG |
|
|
CPT1 |
Sense |
AGACCGTGCGCAAGTACCTAGA |
AF320000 |
|
|
Antisense |
AATAGAGCTGTAGCCGTGGTGC |
|
|
LCAD |
Sense |
ATGCAAGAGCTTCCACAGGAAA |
U21489 |
|
|
Antisense |
CAGAAATCGCCAACTCAGCAAT |
|
|
PDK4 |
Sense |
GTGAACACTCCTTCGGTGCA |
AJ001418 |
|
|
Antisense |
TCAGGCTCTGGATATACCAGCTC |
|
|
UCP2 |
Sense |
TGCCTTCCTTTCTCCGCTTGG |
AF111998 |
|
|
Antisense |
GAAAGGTGCCTCCCGAGATTGGTAG |
|
|
UCP3 |
Sense |
CCGATACATGAACGCTCCCCTA |
AB008216 |
|
|
Antisense |
ATCATCACGTTCCAAGCTCCC |
|
|
PGC-1α |
Sense |
ATACCGCAAAGAGCACGAGAAG |
AF049330 |
|
|
Antisense |
CTCAAGAGCAGCGAAAGCGTCACAG |
|
|
G6PDH |
Sense |
GAACGCAAAGCTGAAGTGAGACT |
Z11911 |
|
|
Antisense |
TCATTACGCTTGCACTGTTGGT |
|
|
rPGC-1α |
Sense |
TTGAAGAGCGCCGTGTGATTTA |
NM_031347 |
|
|
Antisense |
AGTTCTGTCCGCGTTGTGTCAG |
|
|
r36B4 |
Sense |
GACAATGGCAGCATCTACAG |
NM_022402 |
|
|
Antisense |
CAACAGTCGGGTAGC |
|