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Fig. S1. DiI fate mapping of the cardiac NC at stage 17/ (A,B) At stage 27, DiI-labeled NC cells are populating the branchial arches (arrows). Lateral view, dorsal to top, anterior to right. (C,D) Ventral view of a stage 45 embryo showing the restriction of the DiI-positive cardiac NC cells to the aortic arch artery on the right side. (E,F) Transverse sections of a stage 45 embryo. The DiI-positive cardiac NC cells are confined to the aortic sac and arch arteries (arrows) and never populate the cardiac cushions. Brightfield (A,C,E) and fluorescence (B,D,F) images are shown. AA, aortic arch; CC, cardiac cushion; OFT, outflow tract; V, ventricle.
