Files in this Data Supplement:
Fig. S1. MIM is a conserved protein. (A) Amino acid alignment of Xenopus, human, rat and Drosophila proteins demonstrates that MIM is a conserved protein. The red line marks the IMD domain, the green line the SRD domain, the yellow line the PRD domain, and the blue line marks the WH2 domain. The ClustalW 1.83 program was used to obtain the phylogram alignment. (B) Sequence comparison of the individual IMD, SRD, PRD and WM2 domains across species. Note that in Drosophila no clear SRD and PRD sequences were observed by comparison (n.s, not significant).
Fig. S2. Quantification of the subcellular localization studies shown in Fig. 2. (A-C) Quantification of Fig. 2A (A), Fig. 2B (B) and Fig. 2C (C). Blue bar indicates transfected cells with localization of GFP or GFP-MIM to actin stress fibers, whereas the red bar indicates localization of GFP or GFP-MIM to the plasma membrane. Sample size (N) is shown above each bar. Error bars indicate standard deviation of the mean.
