Supplemental Data
Files in this Data Supplement:
- Supplement 4 (.jpg, 663 KB) - Supplemental images to Figure 2.
- Supplement 5 (.jpg, 1.1 MB) - Comparison of CDR surface properties between the model IgG studied in this report and three other IgGs selected from Protein Data Bank (PDB). (A) Computationally modeled variable fragment (Fv) structure of the model IgG used in this study. (B���D) Fv structure models generated by using the structural data available from PDB IDs 3NH7 (1), 3IXT (2), and 3L95 (3). Supplementary References 1. Harth, S., Kotzsch, A., Hu, J., Sebald, W., and Mueller, T. D. (2010) PLoS One 5, e13049 2. McLellan, J. S., Chen, M., Kim, A., Yang, Y., Graham, B. S., and Kwong, P. D. (2010) Nat Struct Mol Biol 17, 248-250 3. Wu, Y., Cain-Hom, C., Choy, L., Hagenbeek, T. J., de Leon, G. P., Chen, Y., Finkle, D., Venook, R., Wu, X., Ridgway, J., Schahin-Reed, D., Dow, G. J., Shelton, A., Stawicki, S., Watts, R. J., Zhang, J., Choy, R., Howard, P., Kadyk, L., Yan, M., Zha, J., Callahan, C. A., Hymowitz, S. G., and Siebel, C. W. (2010) Nature 464, 1052-1057
- Supplement 3 (.jpg, 2.5 MB) - Supplemental images to Figure 1C.
- Supplement 2 (.jpg, 1.5 MB) - Supplemental images to Figure 1B.
- Supplement 1 (.jpg, 282 KB) - Daily monitoring of cell culture conditions. (A) Cell culture medium pH, (B) viable cell density (VCD), and (C) cell viability were measured daily during the 16-day cell cultivation period.
