BMPs and FGFs target Notch signalling via jagged 2 to regulate tooth morphogenesis and cytodifferentiation

DEV049528 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Expression of Pitx2 in the epithelium of dental recombinants. (A) A tooth epithelium/mesenchyme homochronic recombinant. The red dotted line indicates the border between the epithelium (ep) and mesenchyme (mes). (B) Digoxigenin in situ hybridisation on a cryosection showing epithelial Pitx2 expression in an E13.5 dental epithelium/mesenchyme homochronic recombinant.

• Supplemental Figure S2 -

  Fig. S2. Upregulation of Jag2 expression in recombinants of E12.5 dental epithelium cultured on top of E12.5 dental mesenchyme together with FGF2 beads. The red dotted lines indicate the borders between the epithelium (ep) and mesenchyme (mes). b, FGF2 bead.

• Supplemental Figure S3 -

  Fig. S3. Control beads do not affect epithelial Pitx2 expression in tooth recombinants. (A) An E12.5 tooth epithelium/mesenchyme recombinant cultured in the presence of BSA beads (b). The red dotted line indicates the borders between the epithelium (ep) and mesenchyme (mes). (B) Whole-mount in situ hybridisation. BSA beads do not alter Pitx2 expression in the epithelium of E12.5 tooth recombinants.