A large-scale overexpression screen in Saccharomyces cerevisiae identifies previously uncharacterized cell cycle genes -- Data Supplement - Supplemental Table -- Proceedings of the National Academy of Sciences

Supplementary material for Stevenson et al. (2001) Proc. Natl. Acad. Sci. USA 98 (7), 3946–3951. (10.1073/pnas.051013498)

Table 5.
Effect on cell cycle distribution as determined by flow cytometry

>3% Shift
Shift towards 1C DNA content			Shift towards 2C DNA content
ORF	Description	Notes	ORF	Description	Notes
GSP1	Ran family member; CST17 = "Chromosomal Stability"	E*^†	COF1	Actin binding and severing protein, cofilin; role in cell polarity	E^‡
NOP1	Nucleolar protein, fibrillarin	E*	COS4	COS family of subtelomerically-encoded proteins	NE^§
PHB1	Antiproliferative protein involved in determination of replicative lifespan	NE*	HAC1	Transcription factor that activates the unfolded protein response pathway	NE*
RML2	Mitochondrial ribosomal protein L2	NE*	PTR3	Peptide permease regulator	NE*
RPL9A	Ribosomal protein 9A		SEC17	Required for fusion of vesicles to target membranes; important for degradation of internalized a factor	E^‡¶
RPS12	Ribosomal protein S12	NE^‡	YLL023C	Unknown	NE^‡
SBP1	Single-stranded nucleic acid binding protein	NE*^†	YMR067C	Unknown	NE^§
SHY1	Mitochondrial protein required for respiration	NE*	YNL224C	Unknown	NE^§
STE12	Regulates genes required for mating and filamentous growth	NE*
TDH3	Glyceraldehyde-3-phosphate dehydrogenase	NE^‡
TEF2	Translational elongation factor EF1 a	NE*
TOM22	Component of mitochondrial outer membrane receptor complex	E*^†
YBR230C	Unknown	NE^‡
YGR101W	Unknown	NE^‡
YHR181W	Unknown	NE^§
YMR145C	Mitochondrial NADH dehydrogenase	NE^‡
YPL186C	Unknown	NE^†‡
No reproducible shift in cell cycle profile
ORF	Description	Notes	ORF	Description	Notes
ABP1	May stabilize actin filaments at the bud site	NE*^†¶	PUB1	Poly(A)+ RNA binding protein	NE*^†
ACE2	Transcription factor induced at G₂/M	NE^‡	PUS1	tRNA biosynthesis	NE*^¶
AYR1	1-Acyl-dihydroxyacetone phosphate reductase	NE^§	RHO1	Required for cell cycle progression and polarization	E*^¶
CDC48	ATPase	E*	RNT1	RNA processing	E*
DAM1	Involved in mitotic spindle function	E*	RPP0	60S ribosomal protein R0	NE*
DBP2	RNA helicase of DEAD box family	E*	RPS1B	Ribosomal protein S1	E*
DSK2	Ubiquitin-like protein required for SPB duplication	NE^‡	RPS5	Ribosomal protein S5	E*^†
EGD1	CST25 = "Chromosomal Stability"	NE*^†	RPS6A	Ribosomal protein S6	†
EHT1	Alcohol acyl transferase	NE^‡	SEC23	Important for degradation of internalized a factor	E^‡
ERG2	C-8 sterol isomerase	NE*^†	SFI1	Implicated in progression through G₂	E*^†
ERG6	Ergosterol biosynthesis	NE*^†¶	SHE10	"Sensitivity to High Expression"	NE^†‡¶
FUN21	"Function Unknown Now"	NE*	SKN7	Transcription factor involved in G₁ control	NE*
FUN30	SNF2 family protein involved in resistance to UV	NE*	SSE1	Hsp70 family heat shock protein	NE*
GIS1	DNA damage responsive repressor of PHR1	NE*	STO1	Transcriptional activator of glycolytic genes	NE*
GLN1	Glutamine synthetase	NE*	STP3	tRNA splicing and branched chain amino acid uptake	NE^‡
GLC7	Protein phosphatase type I; required for mitosis	E*^¶	UME6	Required for meiosis and ascus formation	NE^‡
HLJ1	DnaJ homologue	NE^†‡	VPS4	Vacuolar sorting; "CDC One Suppressor"	NE^‡
HMO1	Role in chromatin/chromosome structure	NE^*	YRB2	Nuclear-cytoplasmic transport	NE*^†
HSN1	Vesicular transport	NE*^†	YCL056C	Unknown	NE^‡
ILS1	Isoleucyl t-RNA synthetase; ts mutants arrest early G₁	E*	YDR470C	Unknown	NE*^§\|\|
KAP95	Karyopherin b ; nuclear import	E^†‡	YDR514C	Unknown	NE^§
KSP1	Ser/thr protein kinase	NE*^†	YERO53C	Unknown	NE^‡
LPP1	Role in phospholipid metabolism and cell signaling	NE*	YGL068W	Putative mitochondrial ribosomal protein	E^§
MDM10	Involved in coupling mitochondria to cytoskeleton	NE*	YGL224C	Unknown	NE^‡
MRPL16	Mitochondrial ribosomal protein	NE*	YGR026W	Unknown	NE^‡
MRS1	Involved in mitochondrial RNA splicing	NE*^†	YHR162W	Unknown	NE^†‡
NHP6A	Affects CLN1 gene transcription	NE*^†¶	YHR192W	Unknown	NE^§
NPL3	Nuclear/cytoplasmic transport; RNA processing	E*	YKR100C	Unknown	NE^†§¶
NSR1	NLS-binding protein	NE*^¶	YLR253W	Unknown	NE^‡
NTF2	Nuclear transport factor	E*^†	YOR271C	Possible member of MCF	NE^†§
OPI1	Negative regulator of phospholipid biosynthesis	NE*	YPL063W	Unknown	E^†§
PET9	ADP/ATP carrier protein of MCF	NE*^†	YPR013C	Putative transcription factor	NE^§
PEX11	Peroxisomal biogenesis protein; null has multiple buds	NE*	YPR125W	Unknown	NE^‡
PIS1	Phosphatidyl inositol synthase	E*^†	YPR128C	Putative member of MCF	NE^‡
POP1	tRNA maturation and rRNA processing	E*	YCL016C	Unknown	NE^†‡
POR1	Outer mitochondrial membrane porin	NE*^†

Bold
, previously implicated in cell cycle; NE, nonessential gene; E, essential gene; *, published; ‡, Yeast Deletion Project; §, this study; ¶, reported in other overexpression screens; †, variable results upon overexpression; ||, deletion is slow-growing.