Supplemental Data
Files in this Data Supplement:
- Supplemental Figure 2 (.jpg, 2.0 MB) - Supplemental Figure 1. Polycystin-2 no longer localizes to the primary cilium following Cdc42 and Tuba knockdown. A) Polycystin-2 (red) no longer localizes to the primary cilium, or ciliary area, following Cdc42 and Tuba knockdown in MDCK cells. Bar=5 ��m.
- Supplemental Figure 1 (.jpg, 2.4 MB) - Supplemental Figure 1. Sec8 no longer localizes to the primary cilium following Cdc42 and Tuba knockdown. A) MDCK cells containing stable knockdown of Cdc42 and Tuba were grown on Transwell filters for 14 days. Using an antibody against Sec8 (green), localization of Sec8 was examined by confocal microscopy combined with three-dimensional (3D) reconstruction of the stacked series. Please note that DAPI staining (blue) is at a different level in the cell than staining for Sec8, but is included in the merged figure to delineate individual cells and allow for statistical analysis. Bar=5 ��m. B) Quantification demonstrates that virtually no Sec8 is seen localizing to the ciliary area following Cdc42 knockdown, and significantly less is seen following Tuba knockdown.
