UBC9 Autosumoylation Negatively Regulates Sumoylation of Septins in Saccharomyces cerevisiae

Supplemental Data

Files in this Data Supplement:

• Supplemental table 1 (.pdf, 68 KB) - Yeast Strains
• Supplemental figure 5 (.pdf, 53 KB) - Purification of sumoylated Ubc9.
• Supplemental figure 1 (.pdf, 90 KB) - Purified recombinant proteins used for in vitro sumoylation.
• Supplemental figure 2 (.pdf, 243 KB) - Ubc9K0 still has SUMO-conjugation activity .
• Supplemental figure 6 (.pdf, 76 KB) - Sumoylation levels of septin components Cdc3 and Cdc11 in vivo.
• Supplemental figure 4 (.pdf, 155 KB) - Autosumoylation of Ubc9 negatively regulates sumoylation of substrates.
• Supplemental figure 3 (.pdf, 80 KB) - Ubc9 is modified by SMT3.