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Fig. S1. (A−C) Gal-3 is expressed by vimentin+ (Vim) radial glia (arrow) in the P7 SVZ. Scale bar: 50 µm. (D) Cluster of Gal-3+ vimentin+ cells in the P7 RMS. Scale bar: 10 µm. (E,F) Electron microscopy did not reveal major cytoarchitectural changes in Gal3−/− neuroblasts. Scale bar: 1 µm.
Fig. S2. Loss of Gal-3 disrupts SVZ cytoarchitecture. (A−D) immunohistochemistry shows thickened GFAP+ processes (green) in the ventral SVZ of Gal3-null mice. DAPI nuclear counterstain (blue). Scale bar: 20 µm. (E−J) Low magnification wholemount immunohistochemistery shows a decreased number of acetylated-tubulin+ cilia in Gal3−/− mice compared with wild type. Scale bar: 5 µm. The polarity of cilia (acetylated tubulin+) in ependymal cells (β-catenin+) remained unchanged between null and WT mice. Scale bar: 5 µm.
Fig. S3. Gal-3 loss does not affect apoptosis in the SVZ. (A,B) Caspase-3 expression in the SVZ was not altered in Gal-3 mutants. Merged images are shown at higher magnification in A. Scale bar: 5 µm.
Fig. S4. Periglomerular layer interneuron subtypes in WT and Gal3−/− mice. (A,B) Tyrosine hydroxylase+ (TH) neurons. (C,D) Calretinin+ (CR) neurons. (E,F) Calbindin+ (CB) neurons. Scale bar: 40 µm.
Movie 1. Two-photon microscopy showing CTG labeled neuroblast migration in WT slice. The blue and red arrows track two typical motile cells.
Movie 2. Two-photon microscopy showing CTG labeled neuroblast migration in Gal3−/− slice. The blue and red arrows track two typical motile cells.
Movie 3. Gal-3 function blocking antibody decreases explant migration. Compared with control explants, the number of cells and extent of migration is largely decreased in explants cultured in the presence of 2 µg/ml Gal-3 function-blocking antibody. 4 hours and 40 minutes of imaging for each movie starting at 1 hour and 52 minutes after plating explant.
