Phylogenetic and Functional Analysis of Aspergillus fumigatus MGTC, a Fungal Protein Homologous to a Bacterial Virulence Factor -- Gastebois et al. 77 (13): 4700 Data Supplement - Supplemental material -- Applied and Environmental Microbiology

Phylogenetic and Functional Analysis of Aspergillus fumigatus MGTC, a Fungal Protein Homologous to a Bacterial Virulence Factor
Appl. Environ. Microbiol. Gastebois et al. 77: 4700

Supplemental material

Files in this Data Supplement:

Supplemental file 1 - Genome organization of the AfuMGTC region and comparison with N. fischeri (Fig. S1).
Zipped PowerPoint file, 233K.
Supplemental file 2 - Restriction map of the AfuMGTC deletion constructs and cloning steps during AfuMGTC deletion and integration of the hygromycin resistance marker at the AfuMGTC locus; Southern blot analysis of the Δku80 and mtgC mutant strains; AfuMGTC after RNA extraction in the Δku80 and mgtC mutants and the complemented mgtC::MGTC strain using primers MgtCcomp1 and MgtCcomp2 (Fig. S2).
Zipped PowerPoint file, 263K.
Supplemental file 3 - Virulence of the A. fumigatus parental strain, mgtC mutant, and mgtC::MGTC strain in mice; conidial survival (CFU) of the parental strain, mgtC mutant, and mgtC::MGTC strain in mice infected by 6 × 10⁷ conidia after bronchoalveolar washing (Fig. S3).
MS PowerPoint file, 45K.
Supplemental file 4 - Legends to Fig. S1 to S3.
MS Word document, 32K.