Enhancing the Stress Tolerance and Virulence of an Entomopathogen by Metabolic Engineering of Dihydroxynaphthalene Melanin Biosynthesis Genes
Appl. Environ. Microbiol. Tseng et al. 77: 4508

Supplemental material

Files in this Data Supplement:

  • Supplemental file 1 - Supplemental Materials and Methods, including cloning and characterization of melanin biosynthesis genes and transformation shuttle vector construction.
    MS Word document, 42K.
  • Supplemental file 2 - Map of the constructed shuttle vectors for DHN-melanin biosynthetic genes transformation (Fig. S1).
    Zipped MS Word document, 147K.
  • Supplemental file 3 - Thin-layer chromatography of culture extracts from Colletotrichum lagenarium, Alternaria alternata, and Metarhizium anisopliae (Fig. S2).
    Zipped MS Word document, 33K.
  • Supplemental file 4 - LC-MS analysis of DHN-melanin intermediate precursors and shunt products (Fig. S3).
    Zipped MS Word document, 101K.
  • Supplemental file 5 - Spectrometric analysis of melanin (Fig. S4).
    MS Word document, 241K.
  • Supplemental file 6 - Degenerate primers used for cloning DHN-melanin biosynthesis-encoding genes (Table S1).
    Zipped MS Word document, 7K.
  • Supplemental file 7 - Effect of temperature on the growth of the wild type and transformant of Metarhizium anisopliae (Table S2).
    MS Word document, 35K.
  • Supplemental file 8 - Effect of temperature on the conidial germination rate of the wild type and transformant of Metarhizium anisopliae (Table S3).
    MS Word document, 36K.
  • Supplemental file 9 - Effect of water activity on the conidial germination rate of the wild type and transformant of Metarhizium anisopliae (Table S4).
    MS Word document, 38K.