The LC3 recruitment mechanism is separate from Atg9L1-dependent membrane formation in the autophagic response against Salmonella -- Kageyama et al. 22 (13): 2290 Data Supplement - Supplemental Materials -- Molecular Biology of the Cell

Supplemental Materials

This article contains the following supporting material:

Supplemental Materials
Movie01 - GFP-LC3 association with infected S. Typhimurium. MEFs stably expressing GFP-LC3 were infected with S. Typhimurium expressing mCherry for 5 min and images were taken.
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Movie04 - Extended trace of GFP-LC3 association with or negative S. Typhimurium. MEFs stably expressing GFP-LC3 were infected with S. Typhimurium expressing mCherry for 5 min, washed, and then incubated for 1 hour. One GFP-LC3-positive or -negative S. Typhimurium was followed by time-lapse imaging with the automated trace system of MetaMorph until 6 hours post infection over 4 min intervals.
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Movie08 - Localization of Atg9L1-GFP during S. Typhimurium invasion. MEFs stably expressing Atg9L1-GFP were infected with S. Typhimurium expressing mCherry for 5 min and images were taken.
Movie09 - Atg9L1-GFP transiently associated with S. Typhimurium. MEFs stably expressing Atg9L1-GFP were infected with S. Typhimurium expressing mCherry for 5 min and images were taken.
Movie10 - Atg9L1 enclosed S. Typhimurium before LC3 recruitment. MEFs stably expressing Atg9L1-SECFP and YFP-LC3 were infected with S. Typhimurium expressing mRFP for 5 min and images were taken.
Movie11 - Live cell imaging of LC3 and Atg9L1 swapping fluorescent protein. MEFs stably expressing SECFP-YFP and Atg9L1-YFP were infected with S. Typhimurium expressing mRFP for 5 min and images were taken.