Files in this Data Supplement:
Fig. S1. A comparison of the X. laevis snail1 and snail2 MOs with the corresponding sites in the snail2 and snail1 RNAs.
Fig. S2. snail1 morphant effects on neural crest markers. (A-H) Control (A,D,G) and snail1 morphant (one cell of 2-cell embryos injected with 6 ng/embryo) (B,C,E,F,H) embryos were stained in situ at stage 17/18 for twist1 (A-C), chd7 (D-F) and snail2 (G,H). In the injected embryos, lacZ staining is particularly evident on the right-hand side of the embryos shown in C,E,F,H.
Fig. S3. Later stage, Xbra, Antipodean and pΔN63 myotomal phenotypes. (A-E) Injection (one cell of a 2-cell embryo) of an MO against xbra RNA had little effect on myoD expression (B, versus uninjected in A), whereas the antipodean/vegT MO caused a decrease in myoD expression (C), as expected (see Fukuda et al., 2010). Embryos were fixed and stained at stage 25. Together, the xbra and antipodean/vegT MOs had a similar effect on myoD expression as the antipodean/vegT MO alone (D, uninjected side; D′, injected side). A similar decrease in myoD RNA levels was observed in ΔNp63 RNA-injected embryos (E, injected side).
Fig. S4. snail2, snail1 and twist1 morphant phenotype in Xenopus tropicalis. (A-E) The C2 and C3 blastomeres of 32-cell X. tropicalis embryos were injected with snail2 (B,C), snail1 (D) or twist1 (E) MOs (10 ng/embryo). At stage 18, embryos were fixed and stained in situ for sox9 (A, control uninjected embryo); 60% (n=20) of the snail2 morphant embryos showed a decrease in sox9 expression, whereas only 12% (n=25) of the snail1 and 11% (n=35) of the twist1 morphant embryos displayed a similar decrease in sox9 expression.
