Down-regulation of epithelial cadherin is required to initiate metastatic outgrowth of breast cancer -- Wendt et al. 22 (14): 2423 Data Supplement - Supplemental Materials -- Molecular Biology of the Cell

Supplemental Materials

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Supplemental Materials
Movie01 - Systemically dormant cells form intricate 3D branching structures. Nonmetastatic D2.OR cells were grown for 10 days in compliant 3D culture. Movie is a Z-series (400x) that runs from the top to the bottom of the organoid at a depth of 452 ìm that is comprised of 3.2 ìm sections.
Movie02 - D2.OR cells migrate to form multicellular structures under 3D culture conditions. D2.OR cells (7.5x104 cells/cm2) were plated under 3D culture conditions. The timelapse movie (50x) was acquired over a span of 18 h, with individual frames captured at 10 min intervals.
Movie03 - D2.A1 cells are non-migratory under 3D culture conditions. D2.A1 cells (7.5x104 cells/cm2) were plated under 3D culture. The time-lapse movie (50x) was acquired over a span of 18 h, with individual frames captured at 10 min intervals.