Characterization, Localization, Essentiality, and High-Resolution Crystal Structure of Glucosamine 6-Phosphate N-Acetyltransferase from Trypanosoma brucei -- Mariño et al. 10 (7): 985 Data Supplement

Characterization, Localization, Essentiality, and High-Resolution Crystal Structure of Glucosamine 6-Phosphate N-Acetyltransferase from Trypanosoma brucei
Eukaryotic Cell Mariño et al. 10: 985

Supplemental material

Files in this Data Supplement:

Supplemental file 1 - Legends to supplemental figures.
MS Word document, 25K.
Supplemental file 2 - Fig. S1, Southern blot for detection of TbGNA1 in wild-type T. brucei brucei strain 427, and analytical ultracentrifugation profile of recombinant TbGNA1 at final concentrations of 0.75 and 0.50 mg/ml.
Zipped PDF file, 32K.
Supplemental file 3 - Fig. S2, initial rate versus substrate plots, with inset double reciprocal Lineweaver-Burke plots, for GlcN-6P and acetyl-CoA, respectively.
Zipped PDF File, 31K.
Supplemental file 4 - Fig. S3, subcellular localization of TbGNA1 by immunofluorescence microscopy.
Zipped PDF file, 44K.