High-Temperature Enzymatic Breakdown of Cellulose -- Wang et al. 77 (15): 5199 Data Supplement - Supplemental material -- Applied and Environmental Microbiology

High-Temperature Enzymatic Breakdown of Cellulose
Appl. Environ. Microbiol. Wang et al. 77: 5199

Supplemental material

Files in this Data Supplement:

Supplemental file 1 - Supplemental Materials and Methods describing the following: bioinformatics; gene cloning and recombinant protein production; production and purification of recombinant proteins; a standard cellulase enzyme activity assay; pH optima, temperature optima, and thermostability; a β-D-glucosidase enzyme activity assay; and capillary electrophoresis of oligosaccharides.
MS Word document, 59K.
Supplemental file 2 - Oligonucleotide sequences determined by PCR analysis (Table S1).
MS Word document, 47K.