Table S1 - Summary of CSL sequences used |
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Organism |
Accession no. |
Paralog ID |
CSL class |
DBase |
Notes |
Caenorhabditis elegans |
AAB03859 |
|
M |
GenBank (http://www.ncbi.nlm.nih.gov/sites/entrez?db=Protein&itool=toolbar) |
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Ciona intestinalis |
O76808 |
|
M |
GenBank (http://www.ncbi.nlm.nih.gov/sites/entrez?db=Protein&itool=toolbar) |
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Danio rerio |
NP_942579 |
|
M |
GenBank (http://www.ncbi.nlm.nih.gov/sites/entrez?db=Protein&itool=toolbar) |
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Danio rerio |
XP_697075 |
L |
M |
GenBank (http://www.ncbi.nlm.nih.gov/sites/entrez?db=Protein&itool=toolbar) |
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Drosophila melanogaster |
P28159 |
|
M |
GenBank (http://www.ncbi.nlm.nih.gov/sites/entrez?db=Protein&itool=toolbar) |
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Halocynthia roretzi |
BAA20141 |
|
M |
GenBank (http://www.ncbi.nlm.nih.gov/sites/entrez?db=Protein&itool=toolbar) |
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Homo sapiens |
AAH64976 |
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M |
GenBank (http://www.ncbi.nlm.nih.gov/sites/entrez?db=Protein&itool=toolbar) |
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Homo sapiens |
NP_055091 |
L |
M |
GenBank (http://www.ncbi.nlm.nih.gov/sites/entrez?db=Protein&itool=toolbar) |
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Mus musculus |
NP_033061 |
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M |
GenBank (http://www.ncbi.nlm.nih.gov/sites/entrez?db=Protein&itool=toolbar) |
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Mus musculus |
NP_033062 |
L |
M |
GenBank (http://www.ncbi.nlm.nih.gov/sites/entrez?db=Protein&itool=toolbar) |
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Xenopus laevis |
Q91880 |
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M |
GenBank (http://www.ncbi.nlm.nih.gov/sites/entrez?db=Protein&itool=toolbar) |
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Coprinus cinereus |
CC1G_13688 |
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F1 |
Broad Institute (http://www.broad.mit.edu/annotation/genome/coprinus_cinereus/) |
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Coprinus cinereus |
CC1G_03194 |
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F2 |
Broad Institute (http://www.broad.mit.edu/annotation/genome/coprinus_cinereus/) |
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Cryptococcus neoformans |
CNAG_01173 |
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F2 |
Broad Institute (http://www.broadinstitute.org/annotation/genome/cryptococcus_neoformans/) |
intron 2 manually included into cDNA |
Laccaria bicolor |
EU2.LBSCF0001G04170 |
|
F1 |
DOE Joint Genome Institute (http://genome.jgi-psf.org/Lacbi1/Lacbi1.home.html) |
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Laccaria bicolor |
EU2.LBSCF0005G03540 |
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F2 |
DOE Joint Genome Institute (http://genome.jgi-psf.org/Lacbi1/Lacbi1.home.html) |
N-terminus manually truncated based on homology with C. cinereus and the Kozak sequence |
Malassezia globosa |
XP_001730963 |
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F1 |
GenBank (http://www.ncbi.nlm.nih.gov/sites/entrez?db=Protein&itool=toolbar) |
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Melampsora laricis-populina |
ESTEXT_FGENESH2_PG.C_150143 |
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F2 |
DOE Joint Genome Institute (http://genomeportal.jgi-psf.org/Mellp1/Mellp1.home.html) |
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Phanerochaete chrysosporium |
FGENESH1_PG.C_SCAFFOLD_11000203 |
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F1 |
DOE Joint Genome Institute (http://genome.jgi-psf.org/Phchr1/Phchr1.home.html) |
N-terminus manually truncated, based on aa homology and exon/intron structure of C. cinereus and L. bicolor F1 |
Phanerochaete chrysosporium |
Pc_CSL2 |
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F2 |
DOE Joint Genome Institute (http://genome.jgi-psf.org/Phchr1/Phchr1.home.html) |
not defined in the genome database |
Phycomyces blakesleeanus |
ESTEXT_FGENESHPB_PG.C_10057 |
a |
F1 |
DOE Joint Genome Institute (http://genome.jgi-psf.org/Phybl1/Phybl1.home.html) |
exons 2+3 removed (GenScan prediction) – a splice site mispredicted just before the real protein terminus |
Phycomyces blakesleeanus |
ESTEXT_FGENESHPB_PG.C_340109 |
b |
F1 |
DOE Joint Genome Institute (http://genome.jgi-psf.org/Phybl1/Phybl1.home.html) |
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Phycomyces blakesleeanus |
FGENESHPB_PG.17__92 |
a |
F2 |
DOE Joint Genome Institute (http://genome.jgi-psf.org/Phybl1/Phybl1.home.html) |
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Phycomyces blakesleeanus |
FGENESHPB_PG.2__465 |
b |
F2 |
DOE Joint Genome Institute (http://genome.jgi-psf.org/Phybl1/Phybl1.home.html) |
exons 2+3 manually removed (+ GenScan prediction) – a splice site mispredicted just before the real protein terminus |
Phycomyces blakesleeanus |
ESTEXT_FGENESHPB_PG.C_670002 |
c |
F2 |
DOE Joint Genome Institute (http://genome.jgi-psf.org/Phybl1/Phybl1.home.html) |
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Postia placenta |
ESTEXT_FGENESH3_PG.C_1110045 |
a |
F1 |
DOE Joint Genome Institute (http://genomeportal.jgi-psf.org/Pospl1/Pospl1.home.html) |
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Postia placenta |
ESTEXT_FGENESH3_PG.C_810014 |
b |
F1 |
DOE Joint Genome Institute (http://genomeportal.jgi-psf.org/Pospl1/Pospl1.home.html) |
exon 2 manually extended to correct frame (splicing acceptor site changed), results in extending the N-terminus considerably up to the length of the other F1 paralog |
Postia placenta |
ESTEXT_GENEWISE1.C_160061 |
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F2 |
DOE Joint Genome Institute (http://genomeportal.jgi-psf.org/Pospl1/Pospl1.home.html) |
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Rhizopus oryzae |
RO3G_06953 |
a |
F1 |
Broad Institute (http://www.broadinstitute.org/annotation/genome/rhizopus_oryzae/) |
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Rhizopus oryzae |
RO3G_08863 |
b |
F1 |
Broad Institute (http://www.broadinstitute.org/annotation/genome/rhizopus_oryzae/) |
N-terminus manually extended, based on aa homology with RO3G_13784 and P. blakesleeanus F1 |
Rhizopus oryzae |
RO3G_13784 |
c |
F1 |
Broad Institute (http://www.broadinstitute.org/annotation/genome/rhizopus_oryzae/) |
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Rhizopus oryzae |
RO3G_06481 |
a |
F2 |
Broad Institute (http://www.broadinstitute.org/annotation/genome/rhizopus_oryzae/) |
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Rhizopus oryzae |
RO3G_07636 |
b |
F2 |
Broad Institute (http://www.broadinstitute.org/annotation/genome/rhizopus_oryzae/) |
exon 1 manually extended (different splice site selected) to restore a conserved region |
Rhizopus oryzae |
RO3G_11583 |
c |
F2 |
Broad Institute (http://www.broadinstitute.org/annotation/genome/rhizopus_oryzae/) |
|
Rhizopus oryzae |
RO3G_14587 |
d |
F2 |
Broad Institute (http://www.broadinstitute.org/annotation/genome/rhizopus_oryzae/) |
exon 3 manually extended (different splice site chosen) to restore a conserved region, also based on exon/intron pattern homology with other R. oryzae and P. chrysosporium |
Schizosaccharomyces cryophilus |
Scr_CSL1 |
|
F1 |
Broad Institute (http://www.broadinstitute.org/annotation/genome/schizosaccharomyces_group/) |
GenScan prediction + exon 1 added manually (based on homology with S. octosporus F1 and the existence of introns in other Schizosaccharomyces F1); not defined in the genome database |
Schizosaccharomyces japonicus |
SJAG_04092 |
|
F1 |
Broad Institute (http://www.broadinstitute.org/annotation/genome/schizosaccharomyces_group/) |
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Schizosaccharomyces japonicus |
SJAG_03484 |
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F2 |
Broad Institute (http://www.broadinstitute.org/annotation/genome/schizosaccharomyces_group/) |
N-terminus extended (GenScan prediction) |
Schizosaccharomyces octosporus |
SOCG_03749 |
|
F1 |
Broad Institute (http://www.broadinstitute.org/annotation/genome/schizosaccharomyces_group/) |
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Schizosaccharomyces octosporus |
SOCG_04002 |
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F2 |
Broad Institute (http://www.broadinstitute.org/annotation/genome/schizosaccharomyces_group/) |
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Schizosaccharomyces pombe |
SPCC736.08 |
|
F1 |
GeneDB (http://www.genedb.org/genedb/pombe/) |
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Schizosaccharomyces pombe |
SPCC1223.13 |
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F2 |
GeneDB (http://www.genedb.org/genedb/pombe/) |
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Ustilago maydis |
UM05862 |
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F1 |
Broad Institute (http://www.broadinstitute.org/annotation/genome/ustilago_maydis.2/) |
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Ustilago maydis |
UM06280 |
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F2 |
Broad Institute (http://www.broadinstitute.org/annotation/genome/ustilago_maydis.2/) |
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