Files in this Data Supplement:
Fig. S1. Deletion and mutational analyses of POP-1 and A-P asymmetry. (Associated with Fig. 1.) (A,B) GFP fluorescence in embryos at the 2MS and 2E stage. White lines connect AP sister nuclei in the same focal plane. Embryos derive from worm strains expressing specifically in the EMS lineage either GFP::POP-1 wild type (WT) or one of a series of C-terminal deletions, GFP::POP-1 CΔn, where n denotes the number of C-terminal amino acids deleted (A) and GFP::POP-1 carrying the indicated mutations (B). Only one pair of AP sisters each in embryos expressing CΔ2, CΔ4, CΔ6, CΔ8, CΔ10, T425N, S396A, S403,405,407A, S433A, and S433D, is in the same focal plane. Scale bar:10 µm.
Fig. S2. POP-1 C-terminal 50 amino acids are sufficient for WRM-1 binding. Either GFP-tagged POP-1 388-437 or GFP alone was co-expressed with Myc-tagged WRM-1 in HeLa cells. Immunoprecipitation was performed using anti-Myc antibody and western blots were probed with anti-GFP antibody. Left panel, input; right panel, IP. Arrowhead indicates POP-1 389-438; arrow indicates GFP.
