Supplementary material for Wendt et al. (April 3, 2001) Proc. Natl. Acad. Sci. USA, 10.1073/pnas.071051098
Supplemental Movie 2.
Movie of the heavy chain–heavy chain interactions. This view is perpendicular to the crystal plane. Color scheme: the heavy chain of the "free" myosin head is magenta and that of the "blocked" head is red. For both myosin heads the converter domain is green, the essential light chains are blue, and the regulatory light chains, orange. In addition, loops involved in heavy chain–heavy chain interactions are in black on the "free" head and in yellow on the "blocked" head. "Free" head residues potentially involved in the interaction include 458 (at the end of a disordered loop), loop 167–170, and converter domain loop 746–749 and helix 727–732. "Blocked" head residues include loop 368–379, the myopathy loop 407–417, loop 615–618, and helix 392–398. Displayed in cyan in ball-and-stick format are residues 626–634 of the smooth muscle inhibitory domain and "blocked" head residue 406 and "free" head residue 731. The movie rotates a full 360° about an axis through the heavy chain–heavy chain interface.