Occlusive thrombi arise in mammals but not birds in response to arterial injury: evolutionary insight into human cardiovascular disease

Supplemental materials for: Schmaier et al

Files in this Data Supplement:

• Document 1. Supplemental methods and figures (PDF, 299 KB)
  
  
• Video 1. Adhesion of human platelets during perfusion of whole blood over chicken collagen (MOV, 1.58 MB) -
  PPACK-treated human whole blood was labeled with AP-2 mAb and perfused through a tapered-wall parallel plate flow chamber for 5 min over a chicken collagen-coated glass slide at a shear rate of 1,200 s⁻¹.
  
  
• Video 2. Adhesion of chicken thrombocytes during perfusion of whole blood over chicken collagen (MOV, 3.11 MB) -
  PPACK-treated chicken whole blood was labeled with AP-2 mAb and perfused through a tapered-wall parallel plate flow chamber for 5 min over a chicken collagen-coated glass slide at a shear rate of 1,200 s⁻¹.
  
  
• Video 3. Spreading of a human platelet and a chicken thrombocyte on collagen (MOV, 6.09 MB) -
  This video shows the early stages of attachment and spreading of a human platelet (left) a chicken thrombocyte onto an acid-soluble collagen-coated substrate (100 µg/ml) by RICM. The movie is accelerated 30 times and the time stamp presents min:sec. The scale bar represents 3 µm.
  
  
• Video 4. Adhesion of human platelets following treatment with 10 µM eptifibatide and perfusion of whole blood over chicken collagen (MOV, 3.16 MB) -
  Human whole blood was flowed as described for Video 1 with addition of 10 µM eptifibatide.