LOCUS pCM188 8156 bp DNA circular UNA 06-Jan-2011 DEFINITION FEATURES Location/Qualifiers misc_feature 28..1035 /label=tTA\transactivator promoter 1036..1805 /label=CMV\promoter terminator 1817..2028 /label=ADH1\terminator misc_feature 2034..2133 /label=TetO\operator promoter 2134..2286 /label=CYC1\promoter misc_feature 2289..2342 /label=MCS terminator 2343..2604 /label=CYC1\terminator rep_origin 3020..3031 /label=pMB1\ori CDS complement(3784..4644) /label=AmpR CDS 5081..5883 /label=URA3 rep_origin 6221..6965 /label=ARS1 rep_origin 6966..7942 /label=CEN\4 ORIGIN 1 gaattcttat tacgatcctc gcgcccccta cccaccgtac tcgtcaattc caagggcatc 61 ggtaaacatc tgctcaaact cgaagtcggc catatccaga gcgccgtagg gggcggagtc 121 gtggggggta aatcccggac ccggggaatc cccgtccccc aacatgtcca gatcgaaatc 181 gtctagcgcg tcggcatgcg ccatcgccac gtcctcgccg tctaagtgga gctcgtcccc 241 caggctgaca tcggtcgggg gggccgtcga cagtctgcgc gtgtgtcccg cggggagaaa 301 ggacaggcgc ggagccgcca gccccgcctc ttcgggggcg tcgtcgtccg ggagatcgag 361 caggccctcg atggtagacc cgtaattgtt tttcgtacgc gcgcggctgt acgcggaccc 421 actttcacat ttaagttgtt tttctaatcc gcatatgatc aattcaaggc cgaataagaa 481 ggctggctct gcaccttggt gatcaaataa ttcgatagct tgtcgtaata atggcggcat 541 actatcagta gtaggtgttt ccctttcttc tttagcgact tgatgctctt gatcttccaa 601 tacgcaacct aaagtaaaat gccccacagc gctgagtgca tataatgcat tctctagtga 661 aaaaccttgt tggcataaaa aggctaattg attttcgaga gtttcatact gtttttctgt 721 aggccgtgta cctaaatgta cttttgctcc atcgcgatga cttagtaaag cacatctaaa 781 acttttagcg ttattacgta aaaaatcttg ccagctttcc ccttctaaag ggcaaaagtg 841 agtatggtgc ctatctaaca tctcaatggc taaggcgtcg agcaaagccc gcttattttt 901 tacatgccaa tacaatgtag gctgctctac acctagcttc tgggcgagtt tacgggttgt 961 taaaccttcg attccgacct cattaagcag ctctaatgcg ctgttaatca ctttactttt 1021 atctaatcta gacatatgaa ttaattcggg ccgcggaggc tggatcggtc ccggtgtctt 1081 ctatggaggt caaaacagcg tggatggcgt ctccaggcga tctgacggtt cactaaacga 1141 gctctgctta tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg 1201 gagttgttac gacattttgg aaagtcccgt tgattttggt gccaaaacaa actcccattg 1261 acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa accgctatcc acgcccattg 1321 atgtactgcc aaaaccgcat caccatggta atagcgatga ctaatacgta gatgtactgc 1381 caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg 1441 tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg 1501 gcagtttagc gtaaatactc cacccattga cgtcaatgga aagtccctat tggcgttact 1561 atgggaacat acgtcattat tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc 1621 gggccattta ccgtaagtta tgtaacgcgg aactccatat atgggctatg aactaatgac 1681 cccgtaattg attactatta ataactagtc aataatcaat gtcaacatgg cggtaatgtt 1741 ggacatgagc caatataaat gtacatatta tgatatggat acaacgtatg caatgggcca 1801 agctcctcga gtaattcgcg ccacttctaa ataagcgaat ttcttatgat ttatgatttt 1861 tattattaaa taagttataa aaaaaataag tgtatacaaa ttttaaagtg actcttaggt 1921 tttaaaacga aaattcttgt tcttgagtaa ctctttcctg taggtcaggt tgctttctca 1981 ggtatagcat gaggtcgctc ttattgacca cacctctacc ggcagatcaa ttcctcgatc 2041 cctatcagtg atagagagtc gacaaagtcg agtttctcga tccctatcag tgatagagag 2101 tcgacaaagt cgagtttctc gatcgagacc actgcatgca tgtgctctgt atgtatataa 2161 aactcttgtt ttcttctttt ctctaaatat tctttcctta tacattaggt cctttgtagc 2221 ataaattact atacttctat agacacgcaa acacaaatac acacactaaa ttaccggatc 2281 aattcggggg atccgtttaa acaggcctgt taacatcgat agcggccgct agggccctgc 2341 aggagggccg catcatgtaa ttagttatgt cacgcttaca ttcacgccct ccccccacat 2401 ccgctctaac cgaaaaggaa ggagttagac aacctgaagt ctaggtccct atttattttt 2461 ttatagttat gttagtatta agaacgttat ttatatttca aatttttctt ttttttctgt 2521 acagacgcgt gtacgcatgt aacattatac tgaaaacctt gcttgagaag gttttgggac 2581 gctcgaaggc tttaatttgc ggccaagctt ggcgtaatca tggtcatagc tgtttcctgt 2641 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa 2701 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc 2761 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag 2821 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt 2881 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 2941 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 3001 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 3061 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 3121 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 3181 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct 3241 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 3301 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 3361 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 3421 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat 3481 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 3541 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 3601 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 3661 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 3721 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 3781 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 3841 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 3901 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 3961 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 4021 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 4081 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 4141 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 4201 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 4261 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 4321 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 4381 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt 4441 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 4501 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 4561 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 4621 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 4681 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 4741 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat 4801 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcaag aattagcttt 4861 tcaattcaat tcatcatttt ttttttattc ttttttttga tttcggtttc tttgaaattt 4921 ttttgattcg gtaatctccg aacagaagga agaacgaagg aaggagcaca gacttagatt 4981 ggtatatata cgcatatgta gtgttgaaga aacatgaaat tgcccagtat tcttaaccca 5041 actgcacaga acaaaaacat gcaggaaacg aagataaatc atgtcgaaag ctacatataa 5101 ggaacgtgct gctactcatc ctagtcctgt tgctgccaag ctatttaata tcatgcacga 5161 aaagcaaaca aacttgtgtg cttcattgga tgttcgtacc accaaggaat tactggagtt 5221 agttgaagca ttaggtccca aaatttgttt actaaaaaca catgtggata tcttgactga 5281 tttttccatg gagggcacag ttaagccgct aaaggcatta tccgccaagt acaatttttt 5341 actcttcgaa gacagaaaat ttgctgacat tggtaataca gtcaaattgc agtactctgc 5401 gggtgtatac agaatagcag aatgggcaga cattacgaat gcacacggtg tggtgggccc 5461 aggtattgtt agcggtttga agcaggcggc agaagaagta acaaaggaac ctagaggcct 5521 tttgatgtta gcagaattgt catgcaaggg ctccctatct actggagaat atactaaggg 5581 tactgttgac attgcgaaga gcgacaaaga ttttgttatc ggctttattg ctcaaagaga 5641 catgggtgga agagatgaag gttacgattg gttgattatg acacccggtg tgggtttaga 5701 tgacaaggga gacgcattgg gtcaacagta tagaaccgtg gatgatgtgg tctctacagg 5761 atctgacatt attattgttg gaagaggact atttgcaaag ggaagggatg ctaaggtaga 5821 gggtgaacgt tacagaaaag caggctggga agcatatttg agaagatgcg gccagcaaaa 5881 ctaaaaaact gtattataag taaatgcatg tatactaaac tcacaaatta gagcttcaat 5941 ttaattatat cagttattac ccaattctca tgtttgacag cttatcatcg atcgtccaac 6001 tgcatggaga tgagtcgtgg caagaatacc aagagttcct cggtttgcca gttattaaaa 6061 gactcgtatt tccaaaagac tgcaacatac tactcagtgc agcttcacag aaacctcatt 6121 cgtttattcc cttgtttgat tcagaagcag gtgggacagg tgaacttttg gattggaact 6181 cgatttctga ctgggttgga aggcaagaga gccccgagag cttacatttt atgttagctg 6241 gtggactgac gccagaaaat gttggtgatg cgcttagatt aaatggcgtt attggtgttg 6301 atgtaagcgg aggtgtggag acaaatggtg taaaagactc taacaaaata gcaaatttcg 6361 tcaaaaatgc taagaaatag gttattactg agtagtattt atttaagtat tgtttgtgca 6421 cttgcctgca agccttttga aaagcaagca taaaagatct aaacataaaa tctgtaaaat 6481 aacaagatgt aaagataatg ctaaatcatt tggctttttg attgattgta caggaaaata 6541 tacatcgcag ggggttgact tttaccattt caccgcaatg gaatcaaact tgttgaagag 6601 aatgttcaca ggcgcatacg ctacaatgac ccgattcttg ctagcctttt ctcggtcttg 6661 caaacaaccg ccggcagctt agtatataaa tacacatgta catacctctc tccgtatcct 6721 cgtaatcatt ttcttgtatt tatcgtcttt tcgctgtaaa aactttatca cacttatctc 6781 aaatacactt attaaccgct tttactatta tcttctacgc tgacagtaat atcaaacagt 6841 gacacatatt aaacacagtg gtttctttgc ataaacacca tcagcctcaa gtcgtcaagt 6901 aaagatttcg tgttcatgca gatagataac aatctatatg ttgataatta gcgttgcctc 6961 atcaatgcga gatccgttta accggaccct agtgcactta ccccacgttc ggtccactgt 7021 gtgccgaaca tgctccttca ctattttaac atgtggaatt aattctcatg tttgacagct 7081 tatcatcgaa ctctaagagg tgatacttat ttactgtaaa actgtgacga taaaaccgga 7141 aggaagaata agaaaactcg aactgatcta taatgcctat tttctgtaaa gagtttaagc 7201 tatgaaagcc tcggcatttt ggccgctcct aggtagtgct ttttttccaa ggacaaaaca 7261 gtttcttttt cttgagcagg ttttatgttt cggtaatcat aaacaataaa taaattattt 7321 catttatgtt taaaaataaa aaataaaaaa gtattttaaa tttttaaaaa agttgattat 7381 aagcatgtga ccttttgcaa gcaattaaat tttgcaattt gtgattttag gcaaaagtta 7441 caatttctgg ctcgtgtaat atatgtatgc taaagtgaac ttttacaaag tcgatatgga 7501 cttagtcaaa agaaattttc ttaaaaatat atagcactag ccaatttagc acttctttat 7561 gagatatatt atagacttta ttaagccaga tttgtgtatt atatgtattt acccggcgaa 7621 tcatggacat acattctgaa ataggtaata ttctctatgg tgagacagca tagataacct 7681 aggatacaag ttaaaagcta gtactgtttt gcagtaattt ttttcttttt tataagaatg 7741 ttaccaccta aataagttat aaagtcaata gttaagtttg atatttgatt gtaaaatacc 7801 gtaatatatt tgcatgatca aaaggctcaa tgttgactag ccagcatgtc aaccactata 7861 ttgatcaccg atatatggac ttccacacca actagtaata tgacaataaa ttcaagatat 7921 tcttcatgag aatggcccag cgatatatgc ggtgtgaaat accgcacaga tgcgtaagga 7981 gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat 8041 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat 8101 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagt //