LOCUS pCM_HsDHFR 8681 bp DNA circular UNA 13-Jan-2011 DEFINITION FEATURES Location/Qualifiers misc_feature 28..1035 /label=tTA\transactivator promoter 1036..1805 /label=CMV\promoter terminator 1817..2028 /label=ADH1\terminator misc_feature 2034..2133 /label=TetO\operator promoter 2134..2286 /label=CYC1\promoter CDS 2295..2861 /label=HsDHFR terminator 2865..3126 /label=CYC1\terminator rep_origin 3542..3553 /label=pMB1\ori CDS complement(4306..5166) /label=AmpR CDS 5603..6405 /label=URA3 rep_origin 6743..7487 /label=ARS1 rep_origin 7488..8464 /label=CEN\4 ORIGIN 1 gaattcttat tacgatcctc gcgcccccta cccaccgtac tcgtcaattc caagggcatc 61 ggtaaacatc tgctcaaact cgaagtcggc catatccaga gcgccgtagg gggcggagtc 121 gtggggggta aatcccggac ccggggaatc cccgtccccc aacatgtcca gatcgaaatc 181 gtctagcgcg tcggcatgcg ccatcgccac gtcctcgccg tctaagtgga gctcgtcccc 241 caggctgaca tcggtcgggg gggccgtcga cagtctgcgc gtgtgtcccg cggggagaaa 301 ggacaggcgc ggagccgcca gccccgcctc ttcgggggcg tcgtcgtccg ggagatcgag 361 caggccctcg atggtagacc cgtaattgtt tttcgtacgc gcgcggctgt acgcggaccc 421 actttcacat ttaagttgtt tttctaatcc gcatatgatc aattcaaggc cgaataagaa 481 ggctggctct gcaccttggt gatcaaataa ttcgatagct tgtcgtaata atggcggcat 541 actatcagta gtaggtgttt ccctttcttc tttagcgact tgatgctctt gatcttccaa 601 tacgcaacct aaagtaaaat gccccacagc gctgagtgca tataatgcat tctctagtga 661 aaaaccttgt tggcataaaa aggctaattg attttcgaga gtttcatact gtttttctgt 721 aggccgtgta cctaaatgta cttttgctcc atcgcgatga cttagtaaag cacatctaaa 781 acttttagcg ttattacgta aaaaatcttg ccagctttcc ccttctaaag ggcaaaagtg 841 agtatggtgc ctatctaaca tctcaatggc taaggcgtcg agcaaagccc gcttattttt 901 tacatgccaa tacaatgtag gctgctctac acctagcttc tgggcgagtt tacgggttgt 961 taaaccttcg attccgacct cattaagcag ctctaatgcg ctgttaatca ctttactttt 1021 atctaatcta gacatatgaa ttaattcggg ccgcggaggc tggatcggtc ccggtgtctt 1081 ctatggaggt caaaacagcg tggatggcgt ctccaggcga tctgacggtt cactaaacga 1141 gctctgctta tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg 1201 gagttgttac gacattttgg aaagtcccgt tgattttggt gccaaaacaa actcccattg 1261 acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa accgctatcc acgcccattg 1321 atgtactgcc aaaaccgcat caccatggta atagcgatga ctaatacgta gatgtactgc 1381 caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg 1441 tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg 1501 gcagtttagc gtaaatactc cacccattga cgtcaatgga aagtccctat tggcgttact 1561 atgggaacat acgtcattat tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc 1621 gggccattta ccgtaagtta tgtaacgcgg aactccatat atgggctatg aactaatgac 1681 cccgtaattg attactatta ataactagtc aataatcaat gtcaacatgg cggtaatgtt 1741 ggacatgagc caatataaat gtacatatta tgatatggat acaacgtatg caatgggcca 1801 agctcctcga gtaattcgcg ccacttctaa ataagcgaat ttcttatgat ttatgatttt 1861 tattattaaa taagttataa aaaaaataag tgtatacaaa ttttaaagtg actcttaggt 1921 tttaaaacga aaattcttgt tcttgagtaa ctctttcctg taggtcaggt tgctttctca 1981 ggtatagcat gaggtcgctc ttattgacca cacctctacc ggcagatcaa ttcctcgatc 2041 cctatcagtg atagagagtc gacaaagtcg agtttctcga tccctatcag tgatagagag 2101 tcgacaaagt cgagtttctc gatcgagacc actgcatgca tgtgctctgt atgtatataa 2161 aactcttgtt ttcttctttt ctctaaatat tctttcctta tacattaggt cctttgtagc 2221 ataaattact atacttctat agacacgcaa acacaaatac acacactaaa ttaccggatc 2281 aattcgggGG ATCCATGGTT GGTTCTTTGA ATTGTATTGT TGCTGTTTCT CAAAATATGG 2341 GTATTGGTAA GAATGGTGAT TTGCCATGGC CACCATTGAG AAATGAATTC AGATACTTCC 2401 AAAGAATGAC TACTACTTCT TCTGTTGAAG GTAAGCAAAA CTTGGTTATT ATGGGTAAAA 2461 AGACTTGGTT TTCTATTCCA GAAAAGAATA GACCATTGAA GGGTAGAATT AACTTGGTTT 2521 TGTCTAGAGA ATTGAAAGAA CCACCACAAG GTGCTCATTT TTTGTCTAGA TCTTTGGATG 2581 ATGCTTTGAA ATTGACTGAA CAACCAGAAT TGGCTAACAA AGTTGATATG GTTTGGATTG 2641 TTGGTGGTTC TTCTGTTTAC AAAGAAGCTA TGAATCATCC AGGTCATTTG AAATTGTTCG 2701 TTACTAGAAT TATGCAAGAT TTCGAATCTG ATACATTCTT TCCAGAAATT GATTTGGAAA 2761 AGTACAAATT GTTGCCAGAA TATCCAGGTG TTTTGTCTGA TGTTCAAGAA GAAAAGGGTA 2821 TTAAGTACAA GTTTGAAGTT TACGAAAAGA ACGATTGATA ACTGCAGgag ggccgcatca 2881 tgtaattagt tatgtcacgc ttacattcac gccctccccc cacatccgct ctaaccgaaa 2941 aggaaggagt tagacaacct gaagtctagg tccctattta tttttttata gttatgttag 3001 tattaagaac gttatttata tttcaaattt ttcttttttt tctgtacaga cgcgtgtacg 3061 catgtaacat tatactgaaa accttgcttg agaaggtttt gggacgctcg aaggctttaa 3121 tttgcggcca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc 3181 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta 3241 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 3301 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 3361 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 3421 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 3481 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 3541 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 3601 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 3661 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 3721 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 3781 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 3841 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 3901 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 3961 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa 4021 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 4081 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 4141 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 4201 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 4261 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 4321 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 4381 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 4441 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 4501 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 4561 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 4621 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 4681 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 4741 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 4801 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 4861 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 4921 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 4981 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 5041 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 5101 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 5161 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 5221 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 5281 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa 5341 aaataggcgt atcacgaggc cctttcgtct tcaagaatta gcttttcaat tcaattcatc 5401 attttttttt tattcttttt tttgatttcg gtttctttga aatttttttg attcggtaat 5461 ctccgaacag aaggaagaac gaaggaagga gcacagactt agattggtat atatacgcat 5521 atgtagtgtt gaagaaacat gaaattgccc agtattctta acccaactgc acagaacaaa 5581 aacatgcagg aaacgaagat aaatcatgtc gaaagctaca tataaggaac gtgctgctac 5641 tcatcctagt cctgttgctg ccaagctatt taatatcatg cacgaaaagc aaacaaactt 5701 gtgtgcttca ttggatgttc gtaccaccaa ggaattactg gagttagttg aagcattagg 5761 tcccaaaatt tgtttactaa aaacacatgt ggatatcttg actgattttt ccatggaggg 5821 cacagttaag ccgctaaagg cattatccgc caagtacaat tttttactct tcgaagacag 5881 aaaatttgct gacattggta atacagtcaa attgcagtac tctgcgggtg tatacagaat 5941 agcagaatgg gcagacatta cgaatgcaca cggtgtggtg ggcccaggta ttgttagcgg 6001 tttgaagcag gcggcagaag aagtaacaaa ggaacctaga ggccttttga tgttagcaga 6061 attgtcatgc aagggctccc tatctactgg agaatatact aagggtactg ttgacattgc 6121 gaagagcgac aaagattttg ttatcggctt tattgctcaa agagacatgg gtggaagaga 6181 tgaaggttac gattggttga ttatgacacc cggtgtgggt ttagatgaca agggagacgc 6241 attgggtcaa cagtatagaa ccgtggatga tgtggtctct acaggatctg acattattat 6301 tgttggaaga ggactatttg caaagggaag ggatgctaag gtagagggtg aacgttacag 6361 aaaagcaggc tgggaagcat atttgagaag atgcggccag caaaactaaa aaactgtatt 6421 ataagtaaat gcatgtatac taaactcaca aattagagct tcaatttaat tatatcagtt 6481 attacccaat tctcatgttt gacagcttat catcgatcgt ccaactgcat ggagatgagt 6541 cgtggcaaga ataccaagag ttcctcggtt tgccagttat taaaagactc gtatttccaa 6601 aagactgcaa catactactc agtgcagctt cacagaaacc tcattcgttt attcccttgt 6661 ttgattcaga agcaggtggg acaggtgaac ttttggattg gaactcgatt tctgactggg 6721 ttggaaggca agagagcccc gagagcttac attttatgtt agctggtgga ctgacgccag 6781 aaaatgttgg tgatgcgctt agattaaatg gcgttattgg tgttgatgta agcggaggtg 6841 tggagacaaa tggtgtaaaa gactctaaca aaatagcaaa tttcgtcaaa aatgctaaga 6901 aataggttat tactgagtag tatttattta agtattgttt gtgcacttgc ctgcaagcct 6961 tttgaaaagc aagcataaaa gatctaaaca taaaatctgt aaaataacaa gatgtaaaga 7021 taatgctaaa tcatttggct ttttgattga ttgtacagga aaatatacat cgcagggggt 7081 tgacttttac catttcaccg caatggaatc aaacttgttg aagagaatgt tcacaggcgc 7141 atacgctaca atgacccgat tcttgctagc cttttctcgg tcttgcaaac aaccgccggc 7201 agcttagtat ataaatacac atgtacatac ctctctccgt atcctcgtaa tcattttctt 7261 gtatttatcg tcttttcgct gtaaaaactt tatcacactt atctcaaata cacttattaa 7321 ccgcttttac tattatcttc tacgctgaca gtaatatcaa acagtgacac atattaaaca 7381 cagtggtttc tttgcataaa caccatcagc ctcaagtcgt caagtaaaga tttcgtgttc 7441 atgcagatag ataacaatct atatgttgat aattagcgtt gcctcatcaa tgcgagatcc 7501 gtttaaccgg accctagtgc acttacccca cgttcggtcc actgtgtgcc gaacatgctc 7561 cttcactatt ttaacatgtg gaattaattc tcatgtttga cagcttatca tcgaactcta 7621 agaggtgata cttatttact gtaaaactgt gacgataaaa ccggaaggaa gaataagaaa 7681 actcgaactg atctataatg cctattttct gtaaagagtt taagctatga aagcctcggc 7741 attttggccg ctcctaggta gtgctttttt tccaaggaca aaacagtttc tttttcttga 7801 gcaggtttta tgtttcggta atcataaaca ataaataaat tatttcattt atgtttaaaa 7861 ataaaaaata aaaaagtatt ttaaattttt aaaaaagttg attataagca tgtgaccttt 7921 tgcaagcaat taaattttgc aatttgtgat tttaggcaaa agttacaatt tctggctcgt 7981 gtaatatatg tatgctaaag tgaactttta caaagtcgat atggacttag tcaaaagaaa 8041 ttttcttaaa aatatatagc actagccaat ttagcacttc tttatgagat atattataga 8101 ctttattaag ccagatttgt gtattatatg tatttacccg gcgaatcatg gacatacatt 8161 ctgaaatagg taatattctc tatggtgaga cagcatagat aacctaggat acaagttaaa 8221 agctagtact gttttgcagt aatttttttc ttttttataa gaatgttacc acctaaataa 8281 gttataaagt caatagttaa gtttgatatt tgattgtaaa ataccgtaat atatttgcat 8341 gatcaaaagg ctcaatgttg actagccagc atgtcaacca ctatattgat caccgatata 8401 tggacttcca caccaactag taatatgaca ataaattcaa gatattcttc atgagaatgg 8461 cccagcgata tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca 8521 ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt 8581 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc 8641 cagggttttc ccagtcacga cgttgtaaaa cgacggccag t //