Evaluation of Two Magnetic-Bead-Based Viral Nucleic Acid Purification Kits and Three Real-Time Reverse Transcription-PCR Reagent Systems in Two TaqMan Assays for Equine Arteritis Virus Detection in Semen
J. Clin. Microbiol. Miszczak et al. 49: 3694

Supplemental Material

Files in this Data Supplement:

  • Supplemental Figure 1 - Tables S1 (Reagents, primer and probe concentrations, and thermocycling conditions used for detection of EAV nucleic acid by rRT-PCR) and S2 (Real-time RT-PCR and VI results)
    Zipped MS Word document, 18K.
  • Supplemental Figure 2 - Fig. S1 (Determination of analytical sensitivity using 10-fold dilutions of IVT RNA)
    Zipped TIF Image file, 2.2 MB.
  • Supplemental Figure 3 - Fig. S2 (Determination of analytical sensitivity using 10-fold dilutions of TCF containing the EAV VB strain)
    Zipped TIF Image file, 3 MB.
  • Supplemental Figure 4 - Legends to Fig. S1 and S2
    MS Word document, 26K.