Supplemental Materials
This article contains the following supporting material:
- Supplemental Materials
- Movie01 - Movie 1. HeLa cells were transfected with control siRNA. Images were acquired every 2 minutes.
- Movie02 - Movie 2. CIT-K is required specifically for midbody abscission in HeLa cells. HeLa cells transfected with CIT-K siRNA undergoing �early fusion� after midbody formation. Images were acquired every 2 minutes.
- Movie03 - Movie 3. CIT-K is required specifically for midbody abscission in HeLa cells. HeLa cells transfected with CIT-K siRNA undergoing �late fusion� after midbody formation. Images were acquired every 2 minutes.
- Movie04 - Movie 4. HeLa cells were transfected with control shRNA. Images were acquired every 2 minutes.
- Movie05 - Movie 5. CIT-K is required specifically for midbody abscission in HeLa cells. HeLa cells transfected with CIT-K shRNA. Images were acquired every 2 minutes
- Movie06 - Movie 6. GPCs cells from wild type mice. Images were acquired every 2 minutes.
- Movie07 - Movie 7. CIT-K is required specifically for midbody abscission in vivo. GPCs cells from CIT-K knockout mice. Images were acquired every 2 minutes.
- Movie08 - Movie 8. CIT-K-Cherry localized properly during cytokinesis. HeLa cells expressing CITK- Cherry undergoing cytokinesis. The movie was produced by merging phase contrast and the corresponding red fluorescence images, acquired every 2 minutes.
- Movie09 - Movie 9. CIT-K overexpression leads to oscillatory movements. HeLa cells expressing CIT- K-Cherry undergoing cytokinesis. Images were acquired every 2 minutes.
- Movie10 - Movie 10. CIT-K overexpression leads to delayed abscission. HeLa cells expressing CITK- Cherry undergoing cytokinesis. Images were acquired every 2 minutes.
- Movie11 - Movie 11. CIT-K overexpression affects the localization of GFP-ceRhoA. HeLa cells cotransfected with CIT- K-Cherry (red) and GFP-ceRhoA (green) undergoing cytokinesis. The movie was produced by merging the phase contrast frames with the corresponding red fluorescence and green fluorescence frames, acquired every 5 minutes.
- Movie12 - Movie 12. CIT-K overexpression affects the localization of GFP-ANLN. HeLa cells cotransfected with CIT- K-Cherry (red) and GFP-ANLN (green) undergoing cytokinesis. The movie was produced by merging the phase contrast frames with the corresponding red fluorescence and green fluorescence frames, acquired every 5 minutes.