Procoagulant activity induced by vascular injury determines contribution of elevated factor VIII to thrombosis and thrombus stability in mice

Supplemental materials for: Machlus et al

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• Figure S1. Elevated FVIII does not increase fibrin(ogen) incorporation into thrombi following FeCl₃ injury (JPG, 530 KB) -
  Representative sections through thrombi formed in the carotid artery after (A) 3-minute or (B) 2-minute FeCl₃ application. The dark region on the exterior of the vein is the site of FeCl₃ application. H&E staining shows regions of protein and packed erythrocytes. Immunohistochemistry for fibrin (59D8) shows weak fibrin staining concentrated primarily at the luminal edge of proteinaceous regions. “No 1°” indicates antibody 59D8 was omitted from the IHC as a negative control. Slides were imaged with a Retiga 400R camera (Qimaging) linked to an Olympus Bx 61 microscope with a 20 U Plan FL N, 0.5 NA objective lens. A computer equipped with Velocity software (v5.5) was used to operate the system. Images were analyzed with Adobe Photoshop CS (v8.0). All images were taken at the same magnification (20×); the micron bar is 31 µm.