Effects on Transcription of Mutations in ygjD, yeaZ, and yjeE Genes, Which Are Involved in a Universal tRNA Modification in Escherichia coli -- Hashimoto et al. 193 (21): 6075 Data Supplement - Supplemental material -- The Journal of Bacteriology

Effects on Transcription of Mutations in ygjD, yeaZ, and yjeE Genes, Which Are Involved in a Universal tRNA Modification in Escherichia coli
J. Bacteriol. Hashimoto et al. 193: 6075

Supplemental material

Files in this Data Supplement:

Supplemental file 1 - Supplemental Materials and Methods; Fig. S1, construction of ygjD(Ts) and nusB deletion mutants; Fig. S2, growth inhibition induced by nusB and rnhA multicopy plasmids; Fig. S3, effects of the ygjD(Ts) and ΔnusB mutations on transcription; Fig. S4, effects of the dnaG multicopy plasmid on growth of the yeaZ(Ts) and yjeE(Ts) mutants; Fig. S5, construction of the DNA fragment used to join the lacZ reporter to the thrA gene; Fig. S6, construction of the strain expressing the thrA′-lacZ gene; Fig. S7, DNA fragments used to join the lacZ reporter to the thr and ilv genes
PDF file, 2.4MB.
Supplemental file 2 - Table S1, genes up-regulated in the ygjD(Ts) mutant at 30°C; Table S2, genes down-regulated in the ygjD(Ts) mutant at 30°C; Table S3, genes up-regulated in the ygjD(Ts) mutant at 37°C; Table S4, genes down-regulated in the ygjD(Ts) mutant at 37°C; Table S5, genes up-regulated in the ΔnusB mutant; Table S6, genes down-regulated in the ΔnusB mutant; Table S7, genes down-regulated in the ygjD(Ts) mutant and up-regulated in the ΔnusB mutant; Table S8, primer sequences; Table S9, list of primers used for lacZ constructs
PDF file, 6.7MB.