Analysis of a Clonal Lineage of HIV-1 Envelope V2/V3 Conformational Epitope-Specific Broadly Neutralizing Antibodies and Their Inferred Unmutated Common Ancestors
J. Virol. Bonsignori et al. 85: 9998

Supplemental material

Files in this Data Supplement:

  • Supplemental file 1 - Fig. S1 (Total IgG concentration from culture supernatants of IgG+ memory B cells.)
    Fig. S2 (Heavy and light chain alignments of nucleotide and amino acid sequences of clonal lineage monoclonal antibodies CH01, CH02, CH03, and CH04 and their putative reverted unmutated ancestor antibodies.)
    Fig. S3 (Phylogenetic tree of the CH01-CH04 monoclonal antibodies.)
    Fig. S4 (Neutralization breadth of CH01-CH04 and PG9/PG16 monoclonal antibodies.)
    Fig. S5 (CH01-CH04 monoclonal antibodies have functional characteristics of PG9/PG16-like V2/V3 quaternary epitope-specific antibodies.)
    Fig. S6 (Heavy-chain sequence analyses of the CH01-CH04 MAbs predict that CH01-CH04 MAbs have structural characteristics similar to those of PG9 and PG16 V2/V3 quaternary epitope-specific MAbs.)
    Fig. S7 (Autoreactivity and polyreactivity of the CH01-CH04, PG9 and PG16 MAbs.)
    Table S1 (Registry of viruses used in the neutralization assay.)
    Table S2 (Chimera neutralization.)
    Table S3 (CH01-CH04 MAb epitope mapping on V2/V3 single point mutants of consensus C virus.)
    PDF file, 1.6MB.