Correlation between Protection against Sepsis by Probiotic Therapy and Stimulation of a Novel Bacterial Phylotype

Supplemental Material

Files in this Data Supplement:

  • Supplemental Figure 1 - Experimental design (Fig. S1).
    MS Word document, 55K.
  • Supplemental Figure 2 - Alignment of the 16S rRNA gene sequence of CRIB with selected reference sequences of closely related Clostridium spp. and environmental clones (Fig. S2).
    MS Word document, 86K.
  • Supplemental Figure 3 - T-RFLP patterns of the duodenal microbiota of 8 representative diseased rats, treated with either placebo or the probiotic mixture (Fig. S3).
    MS Word document, 353K.
  • Supplemental Figure 4 - Representative T-RFLP patterns of the ileal microbiota of healthy control and diseased animals, treated with either placebo or the probiotic mixture (Fig. S4).
    MS Word document, 184K.
  • Supplemental Figure 5 - Correlation between the relative abundance of CRIB determined by T-RFLP analysis (relative peak intensity of T-RF 457 bp) and qPCR analysis (relative abundance of 16S rRNA gene copies amplified with CRIB-specific primers) (Fig. S5).
    MS Word document, 118K.
  • Supplemental Figure 6 - Associations between different measures of disease outcome and the relative ileal abundance of CRIB in diseased rats after 7 days of acute pancreatitis (Fig. S6).
    MS Word document, 331K.