Files in this Data Supplement:
Fig. S1. Type 2 diabetes phenotypes observed are specific to a high carbohydrate diet. (A-D) The carbohydrate requirement for diet-induced fly diabetes is not strict. All sugars tested result in a similar, dose-dependent developmental delay. High sugar food is not protein deficient. Double the peptone and yeast extract was used to compensate for the reduced feeding volume observed in high sucrose-fed larvae. At 1M sucrose, this was lethal; very few larvae survive. Protein replacement (+2xP) rescued neither weight deficits (E) nor hyperglycemia (F) in 0.7M sucrose-reared larvae.
Fig. S2. Experimental design for microarray analysis. Adult Canton-S flies laid embryos on control or high sucrose foods. Shown is a time course in days after egg lay (days AEL). (A) For 12-hour experiments, control larvae were collected as early L3 (within 12 hours of the L2/L3 molt) and transferred either to fresh control food or high sucrose food for 12 more hours and collected for analysis. (B) For lifespan feeding, embryos laid on control or high sucrose foods were raised until wandering L3 stage and collected for analysis.
Table S1. Nutrient content of control and high sucrose, high fat, and high protein foods. We modified Bloomington's semi-defined food to contain only sucrose. Calculations were based on manufacturer's (Becton, Dickinson, and Co.) datasheets.
Table S2. Gene expression changes in control versus high sucrose-fed larvae. Genes with expression changes ≥1.3 fold in either 12 hour high sucrose-fed (12 fold change) or lifespan high sucrose-fed wandering third instar larvae (ls wL3 fold change) were identified. The average differences in probe intensity values (diff) are included as an approximation of the magnitude of expression changes.