Supplemental Material
This article contains the following supporting material:
- Supplemental Material 1
- Movie01 - Movie S1 ALBA3::YFP signal monitored in trypanosomes during the parasite cycle in the fly Each representative form of the parasite cycle found in the digestive tract (1-6) and salivary glands (7 and 8) of the fly infected with AnTat1.1 ALBA3::YFP trypanosomes are shown as consecutive live movies. First, each form is shown by phase contrast and its name indicated, second its ALBA3::YFP fluorescent signal is visualized by adding a green filter and third, DAPI is visualized by adding a blue filter to show DNA content and localization. The stages that are shown are: (1) procyclic form; (2) a mixed field containing the proventricular parasites: mesocyclic (MS), mesocyclic to epimastigote (MS-E) and dividing epimastigote (DE); (3) a form in mesocyclic to epimastigote transition; (4) dividing epimastigote; (5) long epimastigote, (6) short epimastigote; (7) salivary gland epimastigote and (8) metacyclic.
- Movie02 - Movie S2 ALBA3::YFP signal monitored in fields with mixed trypanosome forms in the fly Different fields of mixed trypanosome forms found in the proventriculus of the fly infected with AnTat1.1 ALBA3::YFP trypanosomes are shown as consecutive live movies. First, each form is shown by phase contrast and its name indicated, second its ALBA3::YFP fluorescent signal is visualized and third, DAPI is visualized to show DNA content and localization. Mixed fields contain the following proventricular forms: (1) mesocyclic (MS), mesocyclic to epimastigote (MS-E) and dividing epimastigote (DE); (2) mesocyclic (MS) and mesocyclic to epimastigote (MS-E); (3) a field containing different parasites in mesocyclic to epimastigote transition (MS-E); (4) mesocyclic to epimastigote (MS-E) and dividing epimastigote (DE); (5) mesocyclic (MS), dividing epimastigote (DE) and short epimastigote (SE); (6) mesocyclic (MS) and long epimastigote (LE); (7) mesocyclic (MS) and dividing epimastigote (DE).
- Movie03 - Movie S3 ALBA4::YFP signal monitored in trypanosomes along the parasite cycle in the fly Each representative form of the parasite cycle found in the digestive tract (1-6) and salivary glands (7 and 8) of the fly infected with AnTat1.1 ALBA4::YFP trypanosomes are shown as consecutive live movies. First, each form is shown by phase contrast and its name indicated, second its ALBA3::YFP fluorescent signal is visualized and third, DAPI is visualized to monitor DNA content and localization. The stages that are shown are: (1) procyclic form; (2) mesocyclic form; (3) a mixed field containing the proventricular parasites: mesocyclic (MS) and mesocyclic to epimastigote (MS-E); (4) a mesocyclic to epimastigote transition; (5) long epimastigote; (6) short epimastigote; (7) salivary gland epimastigote and (8) metacyclic.
- Movie04 - Movie S4 AnTat1.1 GFP control and ALBA::GFP over-expression Fields of mesocyclic parasites, as representative form, of the strains AnTat1.1 GFP control, ALBA3::GFP and ALBA4::GFP are shown as consecutive live movies. First, the phase contrast of each field is shown, second the green fluorescent signal is visualized and third, DAPI is visualized by adding a blue filter to show DNA content and localization.
- Movie05 - Movie S5 Mesocyclic to epimastigote transition during ALBA3::GFP over-expression Parasites of the AnTat1.1 strain over-expressing ALBA3::GFP found in the proventriculus of the fly are shown. First, the phase contrast of each field is shown indicating the name of the forms, second, green fluorescent signal of ALBA3::GFP is visualized and third, DAPI is visualized to monitor DNA content and localization. Following forms are shown: (1) a field of mesocyclics; (2) mesocyclic (MS) and mesocyclic to epimastigote (MS-E); (3) mesocyclic to epimastigote; (4-7) four different atypical cells displaying thin cell diameter (typical for MS-E) and a nucleus in a very anterior position (typical for MS).